甲基丙烯酸环氧丙酯诱导的转化成纤维细胞中P^（53）基因改变  

Alteration of P￣(53) Gene in The Transformed Fibroblasts Induced by Glycidyl Methacrylate

作　　者：谭明家[1] 许建宁[1] 李忠生[1] 谢大英[1] 李玉瑞[1] 方福德[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,中国医学科学院基础医学研究所,中国协和医科大学基础医学院

出　　处：《基础医学与临床》1996年第4期288-292,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金

摘　　要：本研究选用人胚肺成纤维细胞的体外转化实验，建立起细胞转化模型。结果表明甲基丙烯酸环氧丙酯可诱导其表型改变，从转化集落分离的细胞经ＡＢＣ－ＥＬＩＳＡ法检测出Ｐ￣（５３）蛋白增高。ＰＣＲ－ＳＳＣＰ分析揭示抑癌基因Ｐ￣（５３）第７外显子发生突变，从ＰＣＲ双链直接测序的结果发现第７外显子的２４７位ＡＡＣ突变为ＡＧＣ，即由Ａｓｎ变为Ｓｅｒ。因此，Ｐ￣（５３）基因的改变可能是甲基丙烯酸环氧丙酯诱导人胚肺成纤维细胞转化的机制之一。he human embryonic lung fibroblasts transformation was tested by Glycidyl Methacrylate(GMA)in vitro,The results indicate that GMA was able to induce phenotype transformation of human embryonic lung fibrohlasts. The transformed colonies exhibited extensive random orienta-tion and crossing-over of the cells,Increased levels of P(53) oncoprotein was observed in the trans-formed cells by the ELISA-ABC methed,Mutation of P(53) gene was detected from transformedcolonies using Single-Strand Conformation Polymorphism(SSCP)and direct DNA sequencing ofexon 5,7 and 8.Exon 7 showed shifted bands in SSCP. Mutation occured in codon 247 in malig-nant transformants,being A to G transition(amino acid changed from Asn to Ser).These resultssuggest that P(53) gene an important role in the human embryonic lung fibroblasts transformation induced by GMA.

关 键 词：化学致癌物 GMA 丙酯 P53蛋白 成纤维细胞 

分 类 号：R730.231.1[医药卫生—肿瘤]