高效液相色谱法研究啶菌噁唑对番茄灰霉病菌麦角甾醇生物合成的影响  

Effect of Novel Fungicide 5-(4-Chloro phenyl)-2,3-dimethyl-3- (pyridine-3)-oxazoline on Ergosterol Biosynthesis in Botrytis Cinerea by High Performance Liquid Chromatography

作　　者：韩平[1] 刘西莉[1] 刘鹏飞[1] 司乃国[2] 

机构地区：[1]中国农业大学农学与生物技术学院植物病理学系,北京100094 [2]沈阳化工研究院生测室,沈阳110021

出　　处：《分析化学》2006年第10期1467-1470,共4页Chinese Journal of Analytical Chemistry

基　　金：国家科技攻关项目(No.2002BA516A08-11);北京市农业技术攻关项目(No.BJNY2005)资助项目

摘　　要：啶菌噁唑是我国研制的一种新型农用杀菌剂。本实验建立了高效液相色谱法定量分析灰霉病菌麦角甾醇含量的方法,研究了该药剂对番茄灰霉病菌麦角甾醇合成的抑制作用。结果表明,此方法具有良好的准确度和精密度,回收率为98.8%;相对标准偏差为3.0%,适用于测定番茄灰霉病菌麦角甾醇含量;番茄灰霉病菌菌丝在不同浓度啶菌噁唑和对照药剂戊唑醇处理下,药剂对其麦角甾醇合成具有明显的抑制作用,其中在0.5、10.0mg/L啶菌噁唑作用下,菌丝麦角甾醇含量分别为1.59(±0.126)和0.10(±0.011)mg/g干重菌丝,与DMIs类杀菌剂戊唑醇处理相比,没有显著性差异;与空白对照相比,抑制率分别达到22.44%和95·12%;回归分析表明,药剂处理浓度对数与麦角甾醇含量抑制率的机率值表现出正相关线性关系。5-（4-chloro phenyl）-2,3-dimethyl-3-（ pyridine-3 ）-oxazoline （ SYP-Z048 ） is a novel fungicide which is developed by Shenyang Research Institute of Chemical Industry of China. The chemical structure is 5-（4-chloro phenyl） -2,3-dimethyl-3-（ pyridine-3 ） -oxazoline. in this paper, a method for quantitative analysis of ergosterol of B. cinerea by a reversed phase high performance liquid chromatography was established and improved. The results showed this method was suitable for the determination of ergosterol in the hyphae of B. cinerea with the good accuracy and precision. The recovery and the correlation coefficient was 98.8% and 3.0% , respectively. The effect of SYP-Z048 on the ergosterol in the hyphae of B. cinerea was studied. The result revealed that SYP-Z048 could significantly inhibited ergosterol synthesis. The contents of ergosterol in hyphae were （1.59 ± 0. 126） and （0.10 ± 0.011 ） mg/g dry weight under the treated dosage of 0.5 and 10.0 mg/L, respectively. The inhibition rate were 22.24% and 95.12% , which had no remarkably different by contrast with the treatment of DMIs fungicide tebuconazole. Regression analysis indicated that there was positive correlation linear relationship between logarithm value of fungicide concentration and odds score of inhibition rate of ergosterol.

关 键 词：啶菌噁唑 液相色谱 麦角甾醇 抑制作用 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]