高效液相色谱-质谱法分析丰城鸡血藤中刺芒柄花素  

Analysis of Formononetin in the Millettla nitida Benth. var. hirsutissima. Z. Wei by HPLC-Mass Spectrometry

作　　者：余燕影[1] 章丽华[1] 曹树稳[2] 

机构地区：[1]南昌大学化学系,江西南昌330047 [2]南昌大学食品科学教育部重点实验室,江西南昌330047

出　　处：《食品科学》2006年第11期415-418,共4页Food Science

基　　金：国家自然科学基金资助项目(20262004;20562008);江西省教育厅资助项目(赣财教[2003]73号)

摘　　要：采用高效液相色谱-质谱法分析丰城鸡血藤中刺芒柄花素。以乙腈—0.1%甲酸为流动相,流速0.8ml/min,经反相色谱柱SpherisordODS(250mm×4mm,5μm)梯度洗脱分离后进行质谱分析,采用正负电喷雾离子源同时进行信号采集。经HPLC/MS定性确证,丰城鸡血藤含有刺芒柄花素。方法的线性范围为6.0～126.0μg/ml,检出限(S/N=3)和定量下限(S/N=10)分别为0.8和2.7μg/ml,加标回收率为99.6%～104.3%。该方法简便、快速、灵敏度高,可用于丰城鸡血藤中刺芒柄花素分析测定。The formononetin in the MiUettla nitida Benth. vat. hirsutissima. Z.Wei was analyzed by HPLC-Mass spectrometry. By means of acetonitrile-0.1% formic acid as mobile phase with flowing rate at 0.8ml/min, exploiting Spherisord ODS（250mm × 4mm, 5μm）with gradient elution, the tandem mass spectral acquisition was done in positive and negative electrospray ionization. The result showed that the plant studied here contained formononetn.The linear range of this method was from 6.0 to 126.0μg/ml. The limit of detection（LOD, S/N=3） and limit of quantity（LOQ, S/N=10） were 0.8Bg/ml and 2.7μg/ml respectively. The recoveries were between 99.6%- 104.3%. The method can be applied for the determination of formononetin in the Millettla nitida Benth. vat. hirsutissima. Z.Wei because of its briefiness, rapidity, good sensitivity and recovery.

关 键 词：高效液相色谱-质谱 丰城鸡血藤 刺芒柄花素 

分 类 号：O657.63[理学—分析化学]