纳米微粒光敏剂光动力治疗结肠癌的实验研究  

Photosensitizer nanoparticles photodynamic therapy on LOVO human colon cancer xenografts in athymic mice

作　　者：卿三华[1] 李镠洋[1] 盛新华[1] 巴明臣[1] 

机构地区：[1]南方医科大学附属南方医院普通外科,广州510515

出　　处：《中华胃肠外科杂志》2006年第6期530-533,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：广东省科技攻关项目(04B30301018)广东省自然科学基金项目(032902)

摘　　要：目的探讨普通光敏剂profrinⅡ纳米化后光动力疗法(PDT)对结肠癌的生长抑制作用。方法超声乳化法构建profrinⅡ纳米微粒,建立人结肠癌LOVO细胞裸鼠种植瘤模型,把荷瘤裸鼠分为对照组：不注射光敏剂,不用激光照射；纳米微粒光敏剂组：注射profrinⅡ纳米微粒悬液,不用激光照射；普通光敏剂PDT组：注射profrinⅡ后3h,激光照射30 min；纳米微粒光敏剂PDT组：profrinⅡ纳米微粒悬液注射后3 h,激光照射30 min。经小鼠尾静脉注入profrinⅡ纳米微粒(30 mg/kg体重),光敏化3 h后用半导体激光仪垂直照射肿瘤30 min(能量密度9J/cm^2)。然后连续观察肿瘤体积的变化及荷瘤裸鼠生存时间,瘤体苏木精-伊红染色病理分析。结果纳米微粒光敏剂PDT组在治疗后早期产生明显的抑制肿瘤增殖作用,瘤体组织坏死,腺腔样结构解离破坏,微血管坏死,血栓形成。纳米微粒光敏剂PDT组与对照组和纳米微粒光敏剂组相比,延命率提高65．2%和58．3%(P＜0．05),抑瘤率增加87．9%和87．5%(P＜0．05)；与普通光敏剂PDT组相比,延命率提高18．8%(P＜0．05),抑瘤率增加56．0%(P＜0．05)。结论纳米微粒光敏剂PDT可明显抑制裸鼠结肠种植瘤生长,延长荷瘤裸鼠生存期。Objective To evaluate the inhibitory of profrin Ⅱ nanoparticles photodynamic therapy on Lovo human colon cancer xenografts in athymic mice. Methods Profrin Ⅱ nanoparticles were obtained from hypersound emulsification method. LOVO haman colon cancer xenograft were established in athymic mice. Athymic mice were divided into four groups： normal control group, profrin Ⅱ nanoparticles control group, profrin Ⅱ PDT group and profrin Ⅱ nanoparticles PDT group. The animals bearing xenagrafts were treated 30 mg/kg body weight profrin Ⅱ nanoparticles and 3 h later were irradiated with 9 J/cm^2 light from a diode laser. After Profrin Ⅱ nanoparticles PDT, the anti-tumor effect was assessed by messuring tumor valume over a 3 - 4 weeks period, the morphologic changes were observed by macroscopy and microscopy via the histological examination. Results Compared with the control groups, profrin Ⅱ nanoparticles control group, profrin Ⅱ PDT group and profrin Ⅱ nanoparticles-PDT treated tumors had regressed significantly in earlier period with the inhibiting rate being 87.9% （P 〈 0.05）, 87.5% （P 〈 0.05） and 56.0% respectively （P 〈 0.05） In the later period post-PDT, tumors growth resumed with a slower rate. Profrin Ⅱ nanopartieles-PDT polonged the survival time in the treated group with（38.0±6.0） days （P 〈 0.05）. Extensive damage to tumor tissue was found in the earlier period （7 d） post-PDT, whereas in the later period （21 d） post-PDT, islands of vital-looking tumor calls were observed around the damaged tissue. Conclusion Profrin Ⅱ nanoparticles-PDT results in inhibition Lovo colon carcinoma growth in post-PDT earlier period in vivo, and can prolong the survival time of nude mice bearing xenografts significantly, whereas profrin Ⅱ-PDT could not inhibite the growth of colon tumor completely.

关 键 词：光动力疗法 纳米微粒 结肠肿瘤 小鼠 裸 

分 类 号：R735.35[医药卫生—肿瘤]