Trichostatin A Regulates hGCN5 Expression and Cell Cycle on Daudi Cells in vitro  

作　　者：刘红利 陈燕 崔国惠 伍钢 王涛 胡健莉 

机构地区：[1]Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [2]Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第5期534-537,共4页华中科技大学学报（医学英德文版）

基　　金：This project was supported by grant from the National Natural Sciences Foundation of China (No. 30271672).

摘　　要：The expression of human general control of amino acid synthesis protein 5 （hGCN5） in human Burkitt＇s lymphoma Daudi cells in vitro, effects of Trichostatin A （TSA） on cell proliferation and apoptosis and the molecular mechanism of TSA inhibiting proliferation of Daudi cells were investigated. The effects of TSA on the growth of Daudi cells were studied by 3-（4, 5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium （MTT） assay. The effect of TSA on the cell cycle of Daudi cells was assayed by a propidium iodide method. Immunochemistry and Western blot were used to detect the expression of hGCN5. The proliferation of Daudi cells was decreased in TSA-treated group with a 24 h IC50 value of 415.3979 μg/L. TSA induced apoptosis of Daudi cells in a timeand dose-dependent manner. Treatment with TSA （200 and 400 μg/L） for 24 h, the apoptosis rates of Daudi cells were （14.74±2.04） % and （17.63±1.25） %, respectively. The cell cycle was arrested in G0/G1 phase （50, 100 μg/L） and in G2/M phase （200 μg/L） by treatment with TSA for 24 h. The expression of hGCN5 protein in Daudi cells was increased in 24 h TSA-treated group by immunochemistry and Western blot （P〈0.05）. It was suggested that TSA as HDACIs could increase the expression of hGCN5 in Daudi cells, and might play an important role in regulating the proliferation and apoptosis of B-NHL cell line Daudi cells.The expression of human general control of amino acid synthesis protein 5 （hGCN5） in human Burkitt＇s lymphoma Daudi cells in vitro, effects of Trichostatin A （TSA） on cell proliferation and apoptosis and the molecular mechanism of TSA inhibiting proliferation of Daudi cells were investigated. The effects of TSA on the growth of Daudi cells were studied by 3-（4, 5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium （MTT） assay. The effect of TSA on the cell cycle of Daudi cells was assayed by a propidium iodide method. Immunochemistry and Western blot were used to detect the expression of hGCN5. The proliferation of Daudi cells was decreased in TSA-treated group with a 24 h IC50 value of 415.3979 μg/L. TSA induced apoptosis of Daudi cells in a timeand dose-dependent manner. Treatment with TSA （200 and 400 μg/L） for 24 h, the apoptosis rates of Daudi cells were （14.74±2.04） % and （17.63±1.25） %, respectively. The cell cycle was arrested in G0/G1 phase （50, 100 μg/L） and in G2/M phase （200 μg/L） by treatment with TSA for 24 h. The expression of hGCN5 protein in Daudi cells was increased in 24 h TSA-treated group by immunochemistry and Western blot （P〈0.05）. It was suggested that TSA as HDACIs could increase the expression of hGCN5 in Daudi cells, and might play an important role in regulating the proliferation and apoptosis of B-NHL cell line Daudi cells.

关 键 词：Trichostatin A hGCN5 Burkitt＇s lymphoma DAUDI 

分 类 号：R733.1[医药卫生—肿瘤]