鼻咽低分化鳞癌组织中catenins和cyclinD1的表达及其临床意义  

Expression of catenins and cyclin D1 in poorly differentiated squamous cell carcinoma of nasopharynx and their potentially clinical significance

作　　者：沈春英[1] 胡超苏[1] 朱国培[1] 陆洪芬[2] 何少琴[1] 

机构地区：[1]复旦大学附属肿瘤医院放疗科,复旦大学上海医学院肿瘤学系,上海200032 [2]复旦大学附属肿瘤医院病理科,复旦大学上海医学院肿瘤学系,上海200032

出　　处：《中国癌症杂志》2006年第11期968-972,共5页China Oncology

摘　　要：背景与目的:许多研究发现一些分子标志物是重要的预后因素,然而catenins和cyclinD1在鼻咽癌中的价值尚不明确。本研究目的在于探讨鼻咽低分化鳞癌组织中连环蛋白α-、β-和γ-catenin以及细胞周期蛋白cyclinD1的表达,与临床因素及预后的关系。方法:38例鼻咽癌组织的石蜡标本,采用免疫组织化学方法检测α-、β-和γ-catenin和cyclinD1以及肿瘤增殖活性指标ki-67在肿瘤细胞中的表达。结果:本组标本中α-、β-和γ-cate-nin和cyclinD1低表达者略多。它们表达的高低与原发肿瘤T分期、淋巴结有无转移、临床分期和原发肿瘤体积相关性无显著性差异,各标志物的表达与肿瘤增殖活性指标ki-67高低亦未显示明显相关。单因素分析显示,β-cate-nin低表达患者的5年总生存率和无瘤生存率均明显低于高表达者,其值分别为53.2%、29.0%和81.9%、76.0%(P<0.05)。影响患者无瘤生存率的预后因素中,α-catenin的差异达到临界意义(P=0.061)。结论:α-、β-和γ-catenin和cyclinD1在鼻咽癌组织中存在异常表达,初步研究未发现表达异常与肿瘤增殖,分期和转移等存在相关性。仅β-catenin表达降低是影响患者生存率的一个不利预后因素。对这些分子标志物临床意义的检测必须扩大样本量,才有可能为今后的分子靶向治疗提供依据。Background and purpose： Studies have shown that some molecular markers could serve as prognostic factors for nasopharynx carcinoma, but the predictive role of catenins and cyclin D1 remains uncertain for the disease. Our paper is to investigate the expression of catenins（ α-、β- and γ-） and cyclin D1 in nasopharyngeal carcinoma as well as to analysis their relation to clinic factors and prognosis. Methods： We retrieved 38 paraffin-embedded specimens of nasopharynx carcinoma, immunohistochemistry was used to examine the expression of α-、β- and γ-catenin , cyclin D1 and tumor proliferation activity marker ki-67. Results： Reduced expression of α-、β- and γ-catenin and cyclin D1 was observed in most of the tumors. Our preliminary study demonstrated that there was no significant correlation between their expression with T- stage, N-stage, clinical stage and primary tumor volume, as well as with ki-67 stain. In unviarance analysis, patients with reduced expression of β-catenin had poorer prognosis than those with high expression, 5 year overall survival and disease free survival rates of these two groups were 53.2%, 29.0% and 81.9%, 76.0%, respectively（ P 〈 0.05）. The significance of different expression of α-catenin was marginal（ P = 0.061） when analyzing its impact on disease free survival rate. Conclusions： There are abnormal expression of α-,β- , γ--catenin and cyclin D1 in nasopharyngeal carcinoma. Our preliminary result shows that no significant correlation is found between their expression and tumor proliferation, clinical stage and metastasis. Reduced expression of β- catenin might be a negative prognostic factor in terms of survival rates. More cases should be further analyzed to clarify the issue.

关 键 词：鼻咽癌 免疫组织化学 连环蛋白 细胞周期蛋白D1 肿瘤增殖活性 

分 类 号：R739.63[医药卫生—肿瘤]