苦荞茎叶粉中总黄酮酶法提取工艺研究  

Extracting technology of total flavones in powder of Fagopyrum tataricum stem and leaf by enzymatic treatment

作　　者：王敏[1] 高锦明[2] 王军[1] 王丽云[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨陵712100 [2]西北农林科技大学理学院,陕西杨陵712100

出　　处：《中草药》2006年第11期1645-1648,共4页Chinese Traditional and Herbal Drugs

基　　金：科技部攻关计划重大项目(2003BA901A19);西安市2003年农业科技攻关计划项目(NG200317)

摘　　要：目的研究苦荞茎叶粉中总黄酮酶法提取工艺。方法苦荞茎叶粉经纤维素酶处理后用水提取总黄酮,研究酶加酶量、解温度、酶解时间和pH值对总黄酮得率的影响。结果酶法提取的最佳工艺条件为酶解温度55℃,加酶量3.0μL,pH值6.5,酶解90min,再在90℃下提取3次,每次30min,总黄酮得率可达1.47%。结论纤维素酶适用于苦荞茎叶粉中总黄酮的辅助提取。Objective To optimize the extracting technology of total flavones in powder of Fagopyrum tataricum stem and leaf by enzymatic treatment. Methods The powder was treated by cellulase before extracted using water, the effects of enzyme dosage, treatment temperature, treatment time, and pH value on the extract rate of total flavones were studied. Results The optimum extracting technology was as follows ： enzymatic treatment temperature ： 55 ℃, enzyme dosage. 3.0μL, pH value ： 6.5, treatment time. 90 min, extracting 3 times at： 90 C for 30 min once. The extract rate of total flavones was 1.47% by this technology. Conclusion Cellulase could be applied in the assisstant extraction of total flavones in powder of F. tataricum stem and leaf.

关 键 词：苦荞茎叶粉 总黄酮 纤维素酶 正交试验 

分 类 号：R281.2[医药卫生—中药学] R284.06[医药卫生—中医学]