‘富有’和‘次郎’甜柿叶片再生植株的研究  

Shoot Regeneration from Leaves of‘Fuyu’and‘Jiro’Persimmon

作　　者：马俊莲[1] 刘恺[2] 张子德[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]保定师范专科学校化学系,河北保定071051

出　　处：《园艺学报》2006年第5期1048-1050,共3页Acta Horticulturae Sinica

基　　金：河北省重点科技攻关项目(04395501D-3)

摘　　要：比较了不同生长调节剂及其种类浓度和培养时间对‘富有’和‘次郎’两个甜柿(DiospyroskakiThunb.)品种的叶片愈伤组织诱导和不定芽再生的影响。TDZ诱导叶片愈伤组织和不定芽再生的能力很强,在含ZT0·5mg·L-1的培养基中,愈伤组织的形成率达96%,愈伤组织不定芽分化率达80%以上,由0·5mg·L-1TDZ诱导形成的富有和次郎愈伤组织,在含有ZT0·5mg·L-1的1/2DKW培养基培养30d,不定芽分化率均达96%以上,平均芽数分别为7·8和5·4,所得再生苗的生根率近75%。Studies were carried out on high-frequency shoot regeneration from leaves of two Japanese non-astringent persimmon. The effects of growth regulator and cultural time on Fuyu and Jiro, the adventitious shoot regeneration from leaves were investigated. The results showed that TDZ was a strong and efficient hormone inducing adventitious shoot and calli formation. The best regeneration was achieved in 1/2DKW ＋ TDZ 0. 5 mg · L^-1 ＋ ZT 1.0 mg · L^-1, in which callus percentage was 96% and shooting was near above 80%. After 30 day culture, the calli with small shooting were transferred to 1/2DKW medium supplemented with 0. 5 mg · L^-1 zeatin and 0. 1 mg · L^-1 IAA. Shooting regeneration rate were more than 96%, and average adventitious shoots per explant were 7.8 and 5.4 respectively for Fuyu and Jiro after 30 days culture. Rooting percentage from regenerated shoots was about 75%.

关 键 词：柿 TDZ 叶片 植株再生 愈伤组织 

分 类 号：S665.2[农业科学—果树学]