Granzyme B在原发及淋巴转移性胃癌细胞中的差异表达及其与肿瘤血管生成的关系  

Differential expression of Granzyme B in tumor cells between primary and metastatic gastric cancer and its potential role in tumor angiogenesis

作　　者：周业江[1] 熊玉霞[2] 易国平[1] 徐亮[1] 夏冬[1] 魏仁智[1] 

机构地区：[1]泸州医学院附属医院普外科,四川省泸州市646000 [2]泸州医学院药理教研室,四川省泸州市646000

出　　处：《世界华人消化杂志》2006年第30期2938-2942,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30672058

摘　　要：目的:探讨胃癌细胞Granzyme B(GrB)表达在肿瘤血管生成和淋巴转移中的作用．方法:以细胞毒T淋巴细胞为内对照,采用免疫组织化学染色S-P法检测35例胃癌及其中23例淋巴转移癌细胞GrB蛋白的表达;同法检测35例胃癌血管内皮生长因子(VEGF)的表达及CD34标记的微血管密度(MVD)．结果:GrB的表达在无淋巴转移的胃癌高于有淋巴转移者,但无显著性差异,但淋巴转移灶的胃癌细胞GrB阳性率较相应的原发肿瘤显著增高(P=0．038);胃癌GrB与VEGF表达的同步率达57．1％,两者表达水平无明显相关性,而GrB表达水平与胃癌MVD呈显著负相关(r=-0．421,P=0．012);GrB阳性胃癌无论其VEGF表达水平如何,其MVD无显著差异; VEGF阳性胃癌MVD与GrB的表达状态明显相关,GrB阳性者MVD显著下降(P=0．023),在VEGF阴性胃癌中也有类似结果;GrB+＆VEGF-表型胃癌MVD最低,VEGF+&GrB-表型胃癌MVD最高,两者有显著性的差异(P= 0．013)．结论:胃癌细胞GrB表达可能有助于抑制肿瘤血管生成,但也可能有助于淋巴转移胃癌细胞的存活．AIM： To investigate the potential role of Granzyme B （GrB） in the biological behavior of gastric cancer, such as angiogenesis and lymphatic metastasis. METHODS： The identification of GrB expression in gastric cancer cells was performed immunohistologically in patients with primary gastric cancers （PGC, n = 35） and lymphoid metastatic gastric cancer （LMGC, n = 23）, and GrB^＋ cycotoxic T lymphocytes （CTL） served as positive internal control.The antibodies recognizing vascular endothelial growth factor （VEGF） and CD34 were employed to detect the VEGF expression and microvascular density （MVD）, respectively. RESULTS： The positive rate of GrB expression was relatively lower in PGC with lymphoid metastasis than that without metastasis, but no significant difference was observed （P = 0.709）. The positive rate of GrB expression in LMGC was significantly higher than that in the corresponding PGC （P = 0.038）. The synchronous expression rate of GrB and VEGF came up to 57.1%, while there was no significant correlation between them. A significant negative correlation was found between the level of GrB expression and MVD （r=-0.421, P = 0.012） in PGC. VEGF expression was showed to have no significant effect on MVD in GrB positive PGC （P = 0.494）. However, the decreased MVD in VEGF^＋ PGC was remarkably associated with GrB expression （P = 0.023）, and similar feature was exhibited in VEGF negative PGC （P = 0.134）. The lowest and the highest MVD were demonstrated in PGC with positive GrB and negative VEGF, and PGC with positive VEGF and negative GrB, respectively （P = 0.013）. CONCLUSION： The over-expression of GrB in gastric cancer cells might contribute to the inhibition of tumor angiogenesis, but also might contribute to the survival potential of diffused tumor cells in lymph gland.

关 键 词：胃癌 颗粒酶B 血管生成 淋巴转移 

分 类 号：R735.2[医药卫生—肿瘤]