低压缺氧对大鼠海马CA1区神经元P2X受体表达的影响  

Effect of hypoxia on the neurons expression of P2X receptors in CA1 subfield of rat hippocampus

作　　者：赵延东[1] 程赛宇[1] 张金海[1] 吴喜贵[1] 杨忠[1] 阮怀珍[1] 

机构地区：[1]第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所重庆400038

出　　处：《第三军医大学学报》2006年第23期2302-2305,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助面上项目(30470557);全军医学科学技术研究"十一五"计划面上项目(06MA196)~~

摘　　要：目的研究P2X受体各亚型在不同年龄大鼠海马CA1区的表达及低压缺氧的影响。方法应用低压缺氧动物模型,结合免疫组化技术,对大鼠海马CA1区P2X受体进行染色和细胞记数,观测P2X2、4、6受体亚型的表达及缺氧的影响。结果P2X2、4、6受体亚型在大鼠海马CA1区有大量的表达,且受体表达量与中枢神经系统的发育有关;低压缺氧使各个受体亚型的表达上调。结论大鼠海马有P2X受体多个亚型的广泛表达,而低压缺氧使大鼠海马CA1区P2X受体的表达增加。Objective To investigate the expression of P2X receptors in CA1 subfield of rat hippocampus and the effect of hypoxia on the expression. Methods We set up the hypoxia animal model of high altitude and observed the expression of P2X receptors by using immunohistochemical staining before and after hypoxia. Results Immunohistochemistry showed that there were several sub-type of P2X receptors expressed in hippocampus CA1 neurons. After long term hypoxia, the expression of these receptors was increased. Conclusion there were abundant of P2X receptors expressed on the hippocampus CA1 neurons. Hypoxia affects the expression P2X receptors.

关 键 词：缺氧 P2X受体 海马 免疫组化 

分 类 号：R-332[医药卫生] R338.26