二氧化碳气腹环境对人卵巢癌细胞系SKOV_3侵袭能力的影响  

Effects of carbon dioxide pneumoperitoneum on the invasive ability of ovarian carcinoma cell line SKOV3

作　　者：唐均英[1] 黄金[1] 姚珍薇[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《第三军医大学学报》2006年第23期2380-2382,共3页Journal of Third Military Medical University

摘　　要：目的研究腹腔镜手术中二氧化碳(CO2)气腹环境对恶性卵巢肿瘤细胞黏附、侵袭能力的影响,探讨恶性肿瘤行腹腔镜手术的安全性。方法体外培养人卵巢癌SKOV3细胞,模拟腹腔镜CO2气腹环境处理肿瘤细胞,噻唑蓝比色(MTT)法和Boyden小室侵袭运动实验检测SKOV3细胞的黏附、运动和侵袭能力;免疫组化和半定量RT-PCR方法检测SKOV3细胞黏附分子CD44及促血管生成因子bFGF的蛋白和mRNA表达。结果与对照组比较,CO2处理组细胞对人工基底膜的黏附力明显增加(P<0.01);处理组细胞运动和侵袭能力明显增强(P<0.01);其CD44和bFGF蛋白分泌和mRNA表达量显著增加(P<0.05)。结论CO2气腹环境能促进体外培养的恶性卵巢肿瘤细胞的侵袭转移,其作用机制可能与其促进卵巢癌细胞异质黏附和运动能力;增加细胞黏附分子和促血管生成因子的表达有关。Objective To investigate the effects and mechanism of carbon dioxide （CO,） pneumoperitoneum on the adhesive, migratory and invasive abilities of ovarian carcinoma cell line SKOV3. Methods SKOV3cells were exposed to an CO2 insufflation medium. The cells＇ adhesive ability to Matrigel was analyzed by MTF assayand, and their migratory and invasive abilities were analyzed through Boyden filters metastasis model. CD44 and bFGF expressions were examined by immunohistochemistry and semi-quantitative RT-PCR. Results Cells exposed to an in vitro pneumoperitoneum demonstrate could significantly increase adhesive, migratory and invasive abilities compared to the control group （P 〈 0.01 ）. The CD44 and bFGF expressions were significantly increased too （P 〈0.05）. Conclusion Carbon dioxide pneumoperitoneum may improve the adhesive, migratory and invasive abilities of ovarian carcinoma cell line in vitro, probably by increasing the expressions of adhesion and invasion relative molecules .

关 键 词：CO2 气腹 卵巢肿瘤 黏附分子 肿瘤侵袭 

分 类 号：R656[医药卫生—外科学] R73-37[医药卫生—临床医学]