蛋白质二硫键异构酶在幽门螺杆菌感染者胃黏膜中的表达及意义  

Expression of protein disulfide isomerase in gastric mucosa infected with Helicobacter pylori

作　　者：王孟春[1] 郭静波[1] 刘明华[1] 方文刚[2] 李岩[1] 

机构地区：[1]中国医科大学附属第二医院消化内科,辽宁省沈阳市110004 [2]中国医科大学发育生物教研室,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2006年第29期2880-2882,共3页World Chinese Journal of Digestology

基　　金：辽宁省教育厅基金;No.202010794~~

摘　　要：目的：了解蛋白质二硫键异构酶(protein disulfide isomerase,PDI)在幽门螺杆菌(H pylon)感染人胃黏膜组织中的表达情况．方法：分别应用半定量RT-PCR方法及Westem blot方法检测感染(n=32)与未感染(n=28)H pylori的人胃黏膜组织中PDI mRNA及蛋白的表达情况．结果：未感染组PDI mRNA及蛋白表达量分别为0．5704±0．0794,0．5198±0．0379,感染组PDI mRNA及蛋白表达量分别为1．0642±0．1533,0．8252±0．0321：两组相比差异显著(P＜0．01)．结论：正常情况下胃黏膜组织有PDI mRNA的表达及蛋白的合成,H pylori感染可使胃黏膜增加PDI mRNA的表达及蛋白的合成．AIM： To study the expression of protein disulfide isomerase （PDI） in gastric mucosa infected with H pylori. METHODS： Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） and Western blot were used to examine the mRNA and protein expression of PDI, respectively, in human gastric mucosa with or without H pylori infection （n = 32 or 28）. RESULTS： The expression amounts of PDI mRNA and protein were 0.5704 ± 0.0794 and 0.5198 ± 0.0379 in the mucosa without H pylori infection, which were significantly lower than those in the ones with H pylori infection （1.0642 ± 0.1533, 0.8252 ± 0.0321, P 〈 0.01）. CONCLUSION： H pylori the transcription of PDI of PDI protein in gastric infection can increase mRNA and synthesis mucosa.

关 键 词：幽门螺杆菌 蛋白质二硫键异构酶 

分 类 号：R573[医药卫生—消化系统]