人体β-珠蛋白基因5’旁侧调控元件与不同发育时期调控因子的相互作用  

INTERACTION OF CIS-REGULATORY ELEMENT IN THE 5' FLANKING SEQUENCE OF HUMAN β-GLOBIN GENE WITH TRANS-ACTING FACTORS AT DIFFERENT DEVELOPMENTAL STAGES

作　　者：陈士友[1] 蒋俶 钱若兰[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,上海200031

出　　处：《实验生物学报》1996年第4期379-384,共6页Acta Biologiae Experimentalis Sinica

基　　金：中国博士后科学基金;中科院上海细胞所所长基金

摘　　要：人β-珠蛋白基因主要在成年期的骨髓中表达,在胎儿肝,成年肝和K 562细胞中则处于关闭状态。凝胶电泳阻抑法分析发现,在人胎儿肝,成年肝及K 562细胞的核蛋白抽提物中存在着不同的与β-珠蛋白基因5'旁侧调控元件(-372到-194 bp)相结合的红系组织特异性的调控因子。竟争试验的结果表明,成年肝和K 562细胞中的调控因子与β-珠蛋白基因5'旁侧调控元件相互作用的方式具有一定的相似性,这两种细胞的调控因子既结合于负调控区(NCR 2)也结合于正调控区,说明两种细胞中β-珠蛋白基因的关闭机制可能是相似的。胎儿肝的核蛋白因子只结合于负调控区,推测在胎儿期存在独特的关闭机制。Human β-globin gene mainly expressed in the adult bone marrow, while not expressed in fetal liver, adult liver and K562 cells. Using gel mobility shift assay, different protein factors binding to the regulatory elements (from - 372 to - 194 bp) in the 5' flanking sequence of human β-globin gene were detected in the nuclear extracts from human fetal liver, adult liver and K 562 cells respectively. Competitive experiments showed that both the protein factors from adult and K562cells not only bind to negative control region 2 (NCR 2. from -372 to -224 bp), but also bind to positive control region (PCR, from -223 to -194bp), suggesting that there may be similar mechanism to silence the expression of human β-globin gene in these two kind of cells. The protein factor in human fetal liver only binds to NCR 2, indicating that there is a particular silencing mechanism for β-globin gene expression in human fetal stage.

关 键 词：细胞 Β-珠蛋白基因 调控元件 调控因子 

分 类 号：Q28[生物学—细胞生物学]