单抗LC-1与针对的人肺腺癌SPC-A-1细胞肿瘤相关抗原复合物的内化研究  

INTERNALIZATION OF TUMOR ASSOCIATED ANTIGEN ON HUMAN LUNG ADENOCARCINOMA CELL LINE SPC-A-1 BY McAb LC-1

作　　者：谢巍 张荣兴[1] 葛锡锐[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,上海200031

出　　处：《实验生物学报》1996年第3期207-219,共13页Acta Biologiae Experimentalis Sinica

基　　金：国家"八五"攻关项目

摘　　要：我们运用抗人肺癌单抗LC-1结合胶体金免疫电镜技术研究了人肺腺癌SPC-A-1细胞表面抗原抗体复合物被内吞的全过程,发现该细胞表面抗原抗体复合物是通过受体介导内吞途径被内化,经多泡体富集后至溶酶体消化降解。此外我们还用流式细胞仪分析了内吞前后该细胞表面抗原量的变化和短期内的恢复情况。LC-1在诱发该细胞表面抗原内化的同时还诱发了它对该核糖体的自噬。Antigenic modulation of tumor cells is a kind of immunophenomenon that the antigenicity of tumor cell surface antigen could be lessened, weakened or completely lost. It is a potential route for tumor cells to escape the immunosurveillance and im-munoattack of the host. One of the means to cause the antigenic modulation is by means of the antibody. In present research, gold labeled monoclonal antibody LC-l, which is raised against human lung cancer in our lab, as a molecular tracer to study the internalization and the subsequent fate of the membrane tumor associatedantigen-LC-1 complex on the SPC-A-1 cell surface has been used. We found that this complex was internalized via the receptor-mediated endocytic pathway and concentrated in the multivesicular bodies and transported to lysosomes for proteoly-sis in the end. Strikingly, we noted that LC-l had induced the autophagocytosis of ribosomes in SPC-A-1 cells in the mean time it induced the internalization of the cell surface antigen cause by internalization and the restoration after antigenic modulation were also analyzed by the fluorescence activated cell sorter (FACS).

关 键 词：肺肿瘤 癌 腺癌 单克隆抗体 癌细胞 肿瘤抗原 

分 类 号：R734.202[医药卫生—肿瘤]