RP-HPLC法同时测定四物合剂中芍药苷和阿魏酸的含量  

RP-HPLC simultaneous determination of contents of paeoniflorin and ferulic acid in Siwu compound preparation

作　　者：孟令丹[1] 陈晓辉[1] 姚燕[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2006年第10期1398-1400,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定四物合剂中芍药苷和阿魏酸含量的高效液相色谱法。方法:采用 Kromasil C_(50)柱(250mm×4.6mm,5μm),流动相为乙腈-甲醇-水-磷酸(10:20:70:0.035),检测波长为230nm,流速为1.0mL·min^(-1)。结果:芍药苷和阿魏酸的线性范围分别为0.0385～0.370mg·mL^(-1)(r=0.9993)和0.00125～0.020mg·mL^(-1)(r=0.9993),平均加样回收率(n=9)分别为99.1%和100.6%。结论:本方法简便、准确,重复性好,为四物合剂的质量评价提供了依据。Objective：To establish an HPLC method for the simultaneous determination of paeoniflorin and ferulic acid in Siwu compound preparation. Methods：The chromatography was performed on a Kromasil C18 column （250 mm ×4.6 mm,5 μm） with aeetonitrile - methanol - water - phosphoric acid （ 10： 20： 70： 0. 035 ） as the mobile phase and the absorbance was monitored at 230 nm. was set at 35 ℃. Results ：The calibration curves of The flow rate was 1.0 mL · min^-1 and the column temperature paeoniflorin and ferulic acid were linear in the range of 0. 0385 -0. 370 mg · mL^-1（r =0. 9993 ）and 0. 00125 -0. 020 mg · mL^-1 （r =0. 9993 ） respectively. The mean recoveries （ n = 9） of them were 99. 1% and 100.6% respectively. Conclusion： This is an accurate and credible quality control method for Siwu compound preparation.

关 键 词：四物合剂 芍药苷 阿魏酸 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]