气相色谱保留时间预测在药品残留溶剂测定中的应用  

Application of predicting retention times in determination of residual solvents in pharmaceuticals

作　　者：秦立[1] 胡昌勤[2] 

机构地区：[1]云南省药品检验所,昆明650011 [2]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2006年第10期1469-1476,共8页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:通过计算预测有机溶剂在等温和程序升温条件下的保留时间,对测定药品残留溶剂时的色谱条件进行优化。方法:首先确定有机溶剂的气相色谱保留值方程,再运用 Excel 表计算有机溶剂在不同柱温条件下的预测保留时间,进而优化色谱条件,缩短分析时间。结果:可以获得良好的预测准确度。结论:保留时间预测应用于药品残留溶剂测定时色谱条件优化,可以减少预试验次数,较快地选择出合适的色谱条件。Objective ：To predict the retention times of organic solvents under isothermal GC and temperature - programmed GC for optimizing the chromatographic condition in determination of residual solvents in pharmaceuticals. Methods：To establishment of the GC retention equations of each organic solvent, according to the equations, an iterative method was used to predict the retention times of each solvent under a different isothermal or temperature - programmed condition,using a worksheet of Excel as tools,then to choose the optimal chromatographic condition to carry out. Results：Accurate prediction was acquired and the analysis time under the optimized chromatographic condition was largely shortened. Conclusions ： Prediction of retention time is useful in optimizing the chromatographic condition in determination of residual solvents in pharmaceuticals.

关 键 词：保留时间预测 残留溶剂 色谱条件优化 

分 类 号：R917[医药卫生—药物分析学]