TRAIL联合放射线诱导人脑胶质瘤细胞株U251凋亡的协同作用研究  

Experimental Study on Synergistic Effect of TRAIL Combined with Radioactive Ray on Human U251 Glioma Cell Lines

作　　者：戴丹菁[1] 谢丛华[1] 张阿丽[1] 邱慧兵[1] 周遵艳[1] 

机构地区：[1]武汉大学中南医院放化疗科,武汉430071

出　　处：《武汉大学学报（医学版）》2006年第6期759-762,共4页Medical Journal of Wuhan University

基　　金：湖北省科技攻关资助项目(编号:301130949)

摘　　要：目的:研究肿瘤坏死因子相关的凋亡诱导配体(TRAIL)对人脑胶质瘤细胞株U251的凋亡诱导作用及与放射线联合应用的协同作用,并探讨不同剂量TRAIL对人脑胶质瘤细胞株U251凋亡率的影响。方法:利用显微镜、流式细胞仪,观察和检测不同剂量TRAIL、放射线、TRAIL加放射线对传代培养的人脑胶质瘤细胞株U251的凋亡诱导作用,比较各组间肿瘤细胞凋亡率的差异。结果:TRAIL作用后镜下可见到人脑胶质瘤细胞株U251凋亡的典型表现,不同剂量TRAIL均有诱导人脑胶质瘤细胞株U251凋亡的作用,联合应用放射线后U251细胞凋亡率显著提高。结论:TRAIL可有效的诱导人脑胶质瘤细胞株U251凋亡,并和放射线有显著协同作用。Objective： To explore the role of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL） and it＇s combined effect with radiation ray agents on human U251 glioma cell. Methods： Human U251 glioma cells suspension were added to each well of 96-well plates. Cells were incubated for 24 hours with TRAIL, and TRAIL combined with radiation ray agents at different concentration. Rates of apoptosis and death of cells were detected by flow cytometry. Results：The typical morphological manifestation of the U251 glioma cells apoptosis induced by TRAIL was observed under the light microscope and transmission electron microscope. The peaks of sub-G1 appeared in histogram of FCM, which showed that the apoptosis rate of the U251 lioma cells increased significantly with the lapse of time. Conclusion： In vitro, TRAIL is an agent for anti-cancer by inducing the apoptosis of tumor cell, and TRAIL can enhance the effect of radiation ray.

关 键 词：人脑胶质瘤 肿瘤坏死因子相关的凋亡诱导配体 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]