刚地弓形虫低毒DX-STAg对鼠小胶质细胞的影响  

Influence of T.gondii(DX strain) Soluble Tachyzoite Antigen(STAg) on Mice Microglia

作　　者：肖睿璟[1] 刘昌璇[1] 杨茹[1] 何玉玲[1] 陈朗[1] 熊洁[1] 周钢[1] 谭锦泉[1] 

机构地区：[1]武汉大学医学院免疫学系,武汉430071

出　　处：《武汉大学学报（医学版）》2006年第6期695-698,726,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:39870674;30572119;30030130;30471509)

摘　　要：目的:初步探讨刚地弓形虫低毒(T.gondii)DX株-速殖体可溶性抗原(STAg)对小胶质细胞的影响。方法:采用流式细胞术,对不同处理组小鼠小胶质细胞及神经元细胞的凋亡情况进行了分析。并应用原位TUNEL法检测了不同处理组小鼠的小胶质细胞凋亡情况。结果:Tp、TpTi(颅内注入T.gondii)鼠小胶质细胞凋亡数均值约为4个/hpf,高于正常鼠。Tp鼠,凋亡神经元占66%。Tp、TpTi鼠小胶质细胞无NO阻断剂(1-NAME)处理:神经元凋亡分别占60%和71%;而Tp、TpTi小胶质细胞有NO阻断剂(1-NAME)处理:神经元凋亡分别占20%和23%。结论:T.gondii低毒DX-STAg刺激后,所产生的小胶质细胞具有神经毒性。且NO抑制剂能显著性阻断Tp、TpTi鼠小胶质细胞对神经元的凋亡效果。Objective. To explore the influence of T. gondii （DX strain） soluble tachyzoite antigen （STAg） on mice microglia. Methods： Flow cytometry was used to detect functions of neurons from NML,Tp and TpTi mice. TUNEL was used to detect functions of microglia. Results： The numbers of apoptotic microglia in cortex in Tp and TpTi mice were about 4/hpf detected by TUNEL assay in vivo. The percen of apoptotic neurons in Tp mice was 66%. The percentage of apoptotic neurons co-cultured with Tp and TpTi microglia （without 1-NAME-treatment） were 60% and 71%, respectively. But the percent of apoptotic neurons co-cultured with Tp and TpTi microglia plus NO inhibitor （with 1-NAME-treatment） were 20% and 23%, respectively. Conclusion. T. gondii- STAg elicits neurotoxic microglia. NO inhibitor N^ω-nitro-l-arginine methyl ester （1-NAME） significantly inhibits apoptotic effect of Tp and TpTi microglia on neurons in vitro.

关 键 词：小胶质细胞 刚地弓形虫 NO抑制剂 神经元 

分 类 号：R392.12[医药卫生—免疫学]