MCP-1在难治性癫痫患者脑组织中的表达  

The expression of MCP-1 in brain tissue of patients with intractable epilepsy.

作　　者：吴原[1] 王学峰[1] 席志芹[1] 肖飞[1] 李劲梅[1] 朱曦[1] 孙纪军[1] 龚云[1] 刘风英[1] 栾国明[2] 王玉平[3] 李云林[3] 张建国[2] 卢涌 李红卫 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室 [2]首都医科大学天坛医院 [3]首都医科大学宣武医院 [4]重庆刑事科学研究所

出　　处：《中国神经精神疾病杂志》2006年第6期532-536,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：卫生部科学研究基金(98-1-354);重庆市卫生局科研基金(编号:01-2-031)资助

摘　　要：目的了解难治性癫癎患者脑组织中单核细胞趋化蛋白-1(monocyte chemoattractant protein-1, MCP-1)的表达,探索其在难治性癫癎发病机制中的作用。方法在基因芯片扫描获阳性结果基础上,用免疫组织化学方法研究40例难治性癫癎患者脑组织中MCP-1的表达,并与对照组进行比较。结果基因芯片扫描显示MCP-1 mRNA表达上调,免疫组化结果进一步证实MCP-1在难治性癫癎患者脑组织中的表达较对照组明显增多(P<0．05)。结论MCP-1在难治性癫癎的发病机制中可能起着重要作用,阻断MCP-1有可能改变难治性癫癎的预后。Objective To investigate the expression and clinical signification of monocyte chemoattractant protein- 1 （ MCP-1 ） in brain tissue of patients with intractable epilepsy, to learn the role of MCP-1 in the forming of intractable epilepsy. Methods 40 patients with intractable epilepsy were studied with cDNA microarray, and immunohistochemistry, and then MCP-1 expression was evaluated by immunohistochemistry, all results were compared with normal control. Results MCP-1 expression in brain tissue of patients with intractable epilepsy increased when they were compared with normal control, cDNA microarray showed MCP-1 mRNA expression increasing （Ratio =7. 171 ）, and immunohistochemistry result confirmed the results （p 〈0.05）. Conclusions The increased expression of MCP-1 suggested that MCP-1 may play important role in the forming of intractable epilepsy, and block up MCP-1 may inhibited the forming of intractable epilepsy.

关 键 词：难治性癫痫 单核细胞趋化蛋白-1 基因芯片 免疫组化 

分 类 号：R742.1[医药卫生—神经病学与精神病学]