左旋咪唑对实验性自身免疫性脑脊髓炎脊髓IFN-γ、TGF-β_1 mRNA表达的影响  

Effects of Levamisole on the Expressions of IFN-γ mRNA and TGF-β_1 mRNA in Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis

作　　者：吴赛珍[1] 郑荣远[1] 卢晔芬[1] 邱伟文[1] 韩钊[1] 张磊[1] 

机构地区：[1]温州医学院附属第一医院神经内科温州医学院实验神经生物学研究所,325000

出　　处：《中国临床神经科学》2006年第6期584-589,共6页Chinese Journal of Clinical Neurosciences

基　　金：国家自然科学基金项目(编号:30470611);浙江省自然科学基金项目(编号:Y204133)

摘　　要：目的:探讨左旋咪唑(LMS)对实验性自身免疫性脑脊髓炎(EAE)脊髓IFN--γ、TGF-β1mRNA表达的影响。方法:用GPSCH免疫Wistar大鼠制作EAE模型,分别在免疫前(LMS1组)、免疫同时(LMS2组)、免疫后(LMS3组)给LMS10mg·kg-1。比较各组行为学和组织学的变化,用RT-PCR测脊髓IFN-和TGF-β1mRNA的表达。结果:LMS1组和LMS2组的行为学表现和炎细胞浸润加重(P<0.01,P<0.05)。LMS3组出现EAE复发。与EAE组比,LMS1组和LMS2组脊髓IFN-mRNA表达升高,TGF-β1mRNA表达降低,但仅LMS2组有统计学意义(P<0.05)。结论:LMS可升高脊髓IFN-mRNA表达,降低TGF-β1mRNA表达,提示LMS对EAE的促发作用可能与Th1/Th2失衡有关。Aim：To study the effects of levamisole （LMS） given in different stages on the expressions of IFN-γ mRNA and TGF-β1 mRNA in spinal cords of rats with experimental autoimmune encephalomyelitis （EAE）. Methods： EAE of Wistar rats was induced by immunized with guinea pig spinal cord homogenate （GPSCH） and completed Freund＇s adjuvant （CFA） emulsion. LMS was given before or simultaneously or after immunization at a dose of 10 mg·kg^-1. The effect of levamisole on the incidence, latency period, clinical score and histological score of EAE rats were compared by methods of quantization. Meanwhile the expressions of IFN-γ mRNA and TGF-β1 mRNA in spinal cords of different groups were compared by reverse transcription-polymerase chain reaction （RT-PCR）. Results： LMS given before immunization （LMS 1） and LMS given at the same time of immunization （LMS2） could obviously decrease the time of reaching max clinical score （P 〈 0.01 ） and increase the max clinical score, the histological score of inflammatory cells infiltrating in central nervous system（CNS）（P 〈 0.05）.LMS2 may boost the incidence （P 〈 0.05）, but the incidence of LMS1, latency period of LMS 1 and LMS2 have no statistical significance when compared with EAE. LMS given discontinuously after immunization（LMS3） caused relapse in EAE rats. Compared with EAE induced previously,EAE relapsed after recovery progressed more quickly and the duration was shorter, but there were not difference of clinical score and histological score between them. Spinal cords from rats of LMS2 and LMS 1 showed increased level of IFN-γ mRNA, while the level of TGF-β1 mRNA was decreased as compared to EAE. But only LMS2 group has statistical significance. Conclusion： LMS given before immunization and at the time of immunization could aggravate EAE. LMS given after immunization caused relapse of EAE. LMS may shift Th1/Th2 cytokine balance and promote EAE.

关 键 词：左旋咪唑 实验性自身免疫性脑脊髓炎 干扰素-Γ 转化生长因子-Β1 逆转录-聚合酶链反应 

分 类 号：R744.5[医药卫生—神经病学与精神病学]