rhG-CSF增加实验性脑缺血周围区的突触囊泡蛋白的表达  

rhG-CSF Enhances the Expression of Synapstophin in Peri-infarct Zone After Focal Cerebral Ischemia

作　　者：于炳新[1] 吕传真[2] 罗玉敏[2] 

机构地区：[1]山东省立医院神经内科,250021 [2]复旦大学华山医院神经科,200040

出　　处：《中国临床神经科学》2006年第6期590-593,共4页Chinese Journal of Clinical Neurosciences

基　　金：上海市科委基金(编号034119036)

摘　　要：目的:研究重组人粒细胞集落刺激因子(rhG-CSF)对脑梗死周围区的突触囊泡蛋白(synapstophin,SYN)表达的影响。方法:用线栓法建立大鼠大脑中动脉缺血再灌注模型,观察rhG-CSF对神经功能缺损评分的影响;应用半定量逆转录-聚合酶链式反应(RT-PCR)方法和免疫组织化学方法分别测定梗死周围区SYN的mRNA和蛋白表达水平的变化。结果:与对照组相比,治疗组的神经功能缺损评分较低,脑缺血周围区SYN的mRNA和蛋白表达较对照组增高。结论:rhG-CSF可以促进脑缺血周围区的突触囊泡蛋白的表达,从而减轻脑缺血再灌注损伤。Aim： To investigate the effects of recombinant human granulocyte colony-stimulating factor （rhG-CSF） on synapstophin（SYN） expression of periinfarction after focal cerebral ischemia. Methods： Focal brain ischemia in rats was performed with the intraluminal filament occlusion. The effects of rfiG-CSF was evaluated by neurological severity score （NSS）. Reverse transcriptional polymerase chain reaction（RT-PCR） and immunohistochemical techniques were used to detect SYN expression in the peri-infarct zone of the brain. Results： Compared with the control group, rhG-CSF significantly reduced the neurological severity scores and increased the mRNA expression of SYN on 14 d and 21d, while the protein expression of SYN was increased by rhG-CSF treatment on 7d and 14 d. Conclusion： The rhG-CSF may enhance SYN expression and thus alleviate the brain damage after ischemia/reperfusion lesion.

关 键 词：重组人粒细胞集落刺激因子 脑梗死 突触囊泡蛋白 

分 类 号：R743[医药卫生—神经病学与精神病学]