广枣总黄酮对大鼠缺血心肌组织蛋白质表达的影响  

Microarray expression analysis identified several key protein candidates as the potential mediators of total flavones of choerospondias axillaries fructus on myocardial protection

作　　者：张琪[1] 杨玉梅[1] 刘凤鸣 宋一亭 

机构地区：[1]包头医学院心血管研究室,内蒙古包头014010 [2]北京亿立生物技术研究所,北京100044 [3]内蒙古中蒙医研究所,内蒙古呼和浩特010020

出　　处：《中国药理学通报》2006年第11期1344-1348,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30160102)

摘　　要：目的探讨广枣总黄酮对缺血心肌的保护作用及其相关蛋白表达谱的改变。方法应用表面增强激光解吸离子化(surface enhanced laser desorption/lon ization,SELD I)蛋白质芯片技术检测了经广枣总黄酮处理后的缺血心肌组织和未经广枣总黄酮处理后的缺血心肌组织中的蛋白质谱。用PHSII-C型蛋白芯片阅读机读取数据并进行比较分析。结果与未经广枣总黄酮处理后的缺血心肌组织相比,有7个蛋白质在经广枣总黄酮处理后的缺血心肌组织中有变化,其中有2个在广枣总黄酮处理后的缺血心肌组织中呈高表达,5个蛋白低表达,其中有4个出现在IMAC3芯片上,1个出现在SAX2芯片上。结论广枣总黄酮在缺血心肌组织中可调节相关蛋白表达谱从而产生保护作用。Ahn To study the molecular basis of protection on myocardial ischemic injuries of total flavonesof choerospondias axiaris fructus （ TFC ）. Methods The microarray analysis for the protein expression inmyocardium provide a strong tool to explore the key protein candidates involved in the pathogenesis of ischemic injury. Surface enhanced laser desorption/ionization （SELDI） mass spectrometry with protein chip IMAC3, SAX2 and NP20 was used to compare the differentially expressed protein in TFC-treated and untreated ischemic myocardium in rats and the results were analysized with Proteinchip Software 3.0. 2. Resuits Seven differentially expressed proteins were indentified in TFC treated myocardium. These differenti-al effects correlated with the expression of five downregulated proteins and two upregulated proteins, and four of them were discovered on the IMAC3 chip and one of them was discovered on the SAX2 chip. Conelusions The myocardial protection of TFC may be mediated by the differential expression of these proteins which could be the key protein candidates for further investigation.

关 键 词：广枣总黄酮 心肌缺血 蛋白质组 

分 类 号：R-332[医药卫生] R282.71