肠包衣地塞米松脂质体壳聚糖胶囊对炎性结肠组织MPO活性的影响  

Study on the effect of myeloperioxidase in rat colitis by oral administration of HPMCP-coated chitosan capsule filled with dexamethasone liposome lyophilized powder

作　　者：李国锋[1] 侯连兵[1] 霍启录[1] 

机构地区：[1]南方医科大学南方医院药学部,广东广州510515

出　　处：《中国药理学通报》2006年第11期1371-1375,共5页Chinese Pharmacological Bulletin

基　　金：广东省自然科学基金资助项目(No020056);广东省医学科研基金资助项目(NoA2002371)

摘　　要：目的评价肠包衣地塞米松(DSP)脂质体冻干剂壳聚糖胶囊对大鼠炎性结肠组织髓过氧化酶(MPO)活性的影响。方法用2,4,6-三硝基苯磺酸钠(TNBS)诱导大鼠结肠炎,建立反应结肠炎症程度的结肠组织MPO浓度的测定方法,观察口服各种DSP制剂对MPO的抑制程度。结果TNBS灌肠后d 5时MPO达到最高值;各种DSP制剂均有降低TNBS诱导的实验性大鼠结肠炎结肠组织MPO的效果,但DSP脂质体壳聚糖胶囊具有最好的抑制作用。结论肠包衣DSP脂质体冻干剂壳聚糖胶囊在治疗结肠炎中可能具有较大的应用前景,值得进一步开发研究。Aim To observe the inhibition of myeloperioxidase （MPO） activity in rat colitis by oral administration of HPMCP-coated chitosan capsule filled with dexamethasone （ DSP ） liposome lyophilized powder. Method The rat colitis was induced with the coloclysis of 2, 4, 6-trinitrobenzene sulfonic acid （TNBS） , and then methods were established for quantifying MPO in colon tissue and the ratio of rat colon weight/rat body weight （C/B）, which were chosen as the index of colitis degree. These indexes were used to evaluate the inhibiting action of various DSP dosage forms on MPO in colon tissue after their oral administration. Re-suits The observed MPO in colon tissue reached the max on the fifth day with the coloclysis of TNBS. All the experimented DSP preparations could effectively inhibit MPO activity, while DSP lyophilized liposome capsule had the most effectiveness. Conclusion DSP lyophilized liposome chitosan capsule may be more helpful in treating colitis than DSP powder chitosan capsule and its other dosage forms. Hence, it is worth studying further.

关 键 词：地塞米松 壳聚糖胶囊 脂质体 髓过氧化酶 肠溶性 

分 类 号：R-332[医药卫生] R322.45