壳聚糖对大鼠Ⅲ度烧伤创面EGF和PDGF表达的影响  

Effect of chitosan on EGF and PDGF in the wound of Ⅲ skin burns in rats

作　　者：廖红[1] 董志[1] 傅洁民[2] 朱毅[3] 朱丽霞[1] 刘全[1] 

机构地区：[1]重庆医科大学药理教研室生物化学和分子药理学重点实验室,重庆400016 [2]重庆医药工业研究院,重庆400061 [3]海南省药品检验所,海南海口570216

出　　处：《中国药理学通报》2006年第11期1375-1378,共4页Chinese Pharmacological Bulletin

摘　　要：目的研究壳聚糖对大鼠Ⅲ度烧伤创面表皮生长因子(EGF)和血小板源性生长因子(PDGF)表达的影响。方法W istar大鼠分为1%壳聚糖组(W/V)、2%壳聚糖组(W/V)、4%壳聚糖组(W/V)、成纤维细胞生长因子(贝复剂)组(阳性对照组)、自然愈合组(模型组),制备成大鼠Ⅲ度烧伤模型,采用免疫组织化学SABC法、原位杂交法及图像分析法检测大鼠Ⅲ度烧伤创面EGF和EGFmRNA的表达;PDGF的活性用L929细胞生物法测定。结果4%壳聚糖组(W/V)对烧伤后7、14 d创面EGF的表达、对烧伤后3、7 d创面EG-FmRNA的表达有明显的提高;对烧伤后3、7、14 d创面PDGF的活性有明显的提高,与自然愈合组相比,差异均有显著性(P<0.05)。结论壳聚糖对大鼠Ⅲ度烧伤创面的EGF表达和PDGF活性有促进作用。Aim To study the effect of chitosan on the expression of epidermal growth factor （EGF） and the activity of platelet-derived growth factor （PDGF） in the wound of Ⅲ skin burns in rats. Methods Wistar rats were randomly divided into 5 groups as follows： 1% chitosan （W/V） group, 2% chitosan （W/V） group, 4% chitosan （W/V） group, bFGF （basic fibroblast growth factor） group and the control group. Rats were made for Ⅲ skin burns. Immunohistochemisty, in situ hybridation and image analysis were employed to detect the expression of EGF and its mRNAin the wound of Ⅲ skin burns in rats; PDGF was determined by bioassay. Results Expression of EGF, EGF mRNA and PDGF in the wound of Ⅲ skin burns in rats using 4% chitosan （W/V） were significantly higher than those of other groups. Conclusion Chitosan could significantly promote expressions of EGF and PDGF during the wound healing of Ⅲ skin burns in rats.

关 键 词：壳聚糖 表皮生长因子 血小板源性生长因子 烧伤 愈合 

分 类 号：R-332[医药卫生] R322.99