血管生成抑制剂NM-3抑制胃癌生长机制研究  

Study of angiogenesis inhibitor NM-3 on the growth of human gastric cancer

作　　者：麻继锋 朱祖明[2] 朱金水[3] 吴霞[1] 

机构地区：[1]马鞍山市中国十七冶医院消化科,安徽马鞍山243000 [2]南京市江宁医院消化科,江苏南京210000 [3]上海交通大学附属第六人民医院消化科,上海200000

出　　处：《皖南医学院学报》2006年第4期265-267,共3页Journal of Wannan Medical College

基　　金：上海市科委自然基金项目(02ZB-14072)

摘　　要：目的:研究血管生成抑制剂NM-3对胃癌生长的影响,探讨其抑制胃癌生长可能存在的分子机制。方法:建立人胃癌裸鼠皮下种植模型。将40只荷瘤裸鼠随机分成4组,种植后第2 d开始分别腹腔内注入生理盐水(2 ml/kg,对照组)、NM-3(剂量分别为10 mg/kg、20 mg/kg、40 mg/kg,治疗组),每周2次,共6周。第7周处死动物,测量皮下肿瘤体积、计算抑瘤率;取瘤组织行免疫组化染色测定微血管密度(MVD)、胃癌细胞凋亡指数(AI)。结果:NM-3剂量为10 mg/kg、20 mg/kg、40 mg/kg的皮下肿瘤体积分别为(915.46±39.66)mm3(、871.26±38.84)mm3(、812.27±32.59)mm3,明显低于生理盐水对照组(1 609.55±41.32)mm3(P<0.05),抑瘤率为43.20%、45.87%、49.53%;和生理盐水组比较,NM-3治疗后肿瘤的微血管密度明显减少,胃癌细胞的凋亡指数显著增加(P均<0.05),且与治疗剂量有关。结论:NM-3可通过抑制肿瘤微血管生成,诱导胃癌细胞凋亡,抑制体内人胃癌生长。Objective：To explore the effect of a novel angiogenesis inhibitor NM-3 on the growth of human gastric carcinoma SGC-7901 cell line in BALB/C nude mice and its potential mechanism. Methods ： The models with human gastric cancer SGC-7901 cell were established by subcutaneous xenotransplantation. The pieces of tumor tissue under the dorsal skin of nude mice were fixed. These nude mice were randomly divided into four groups （ 10 nude mice in each group） ： saline control group, NM-3 treatment group（including 3 subgroups）. From the second day after xenotransplantation, normal sallne（20ml/kg）and NM-3 in a dose of 10 mg/kg, 20 mg/kg and 40 mg/kg were administered i, p, respectively in the involved groups three times a week for 6 weeks. At the end of the sixth week after treatment, the nude mice were sacrificed and the intact tumor tissue was separated immediately, the volume of tumor in mice was measured so as to calculate the tumor growth inhibitive rate. The level of apoptotic index（Al）of gastric cancer cell was assessed by normal staining with HE and the microvascular density（MVD） in tumor tissue was examined by immunohistochemical staining with anti- CD34 monoclonal antibody. Results ： The volume of subcutaneously implanted tumor in nude mice in each group of NM-3 treatment alone was （915.46 ± 39.66）mm^3,（871.26 ± 38.84）mm^3 and （812.27 ± 32.59） mm^3 respectively, which were significantly smaller than that in saline control group[（1 609.55±41.32） mm^3（P〈0.05） ]. The tumour inhibitive rate was 43.20 % , 45.87 % and 49.53 % in NM-3 dose-dependent manner, especially in groups with NM-3 therapy in a dose of 40 mg/kg. The apoptotic index（AI） of gastric cancer cell was obviously increased and microvascular density （MVD） in tumor tissue was significantly decreased in each mouse treated by NM-3 （P 〈 0.05）and was related with NM-3 dosage. Conclusion： A novel angiogeneais inhibitor NM-3 can inhibit the growth of human no-differential gastric carcinoma SGC-79

关 键 词：胃癌 血管生成抑制剂 NM-3 细胞凋亡指数 微血管密度 

分 类 号：R735.2[医药卫生—肿瘤]