小麦1BL/1RS易位系1RS分子标记位点稳定性分析  

Analysis on the Stability of 1RS in the Wheat-rye 1BL/1RS Translocation Lines

作　　者：苏亚蕊[1] 张大乐[1] 高安礼[1] 李玉阁[1] 李锁平[1] 

机构地区：[1]河南大学河南省植物逆境生物学重点实验室,河南开封475001

出　　处：《麦类作物学报》2006年第6期6-10,共5页Journal of Triticeae Crops

基　　金：河南省高校杰出科研人才创新工程项目(2007KYCX009)

摘　　要：为检测小麦1BL/1RS易位系1RS位点的稳定性,利用1RS上11个标记位点的PCR特异引物对21个小麦1BL/1RS易位系进行了分子检测。结果表明,21个1BL/1RS易位系中,66.7%的品种1RS的11个标记位点较为稳定,扩增出标记位点特异性带,但有33.3%的品种部分标记位点出现特异性带的丢失或添加,具有不同程度的位点变异,位点变异率最高达45.5%。对于1RS上的11种PCR特异引物,引物NOR-R1和APR1.3可稳定扩增出黑麦特异带,是在小麦遗传背景中鉴别黑麦1RS较为可靠的分子标记。In order to identify the stability of 1RS in these wheat-rye 1BL/1RS translocation lines, 11 1RS specific primer sets were used to detect the 1RS in 21 wheat-rye 1BL/1RS translocation lines. The results indicated that 66.7% of the 21 wheat-rye 1BL/1RS translocation lines had a stable 1RS and amplified the specific band. However, 33.3% of the lines had some variations, there were some deletions and additions of the specific band in 1RS. The highest variation frequency is 45.5%. In addition,among the 11 1RS specific primers used in the experiment, only the primer sets NOR-R1 and APR 1.3 amplified the special band in all samples, which provided a useful means for molecular tagging of rye chromosomes 1RS in wheat genetic background.

关 键 词：小麦 黑麦 1BL/1RS易位系 分子标记 

分 类 号：S512.1[农业科学—作物学] S330