原发性肝癌癌细胞DNA倍体类型对预后的影响  

The DNA ploidy of cancerous cells in patients with primary liver cancer influences the prognosis

作　　者：晁鹏丽[1] 刘莉莉[1] 张忠英[1] 

机构地区：[1]厦门市厦门大学附属中山医院临床检验中心,316004

出　　处：《实用肝脏病杂志》2006年第6期340-342,共3页Journal of Practical Hepatology

摘　　要：目的探讨原发性肝癌癌细胞DNA倍体类型对肝癌复发及病人生存期的影响。方法利用流式细胞术（FcM）检测原发性肝癌细胞DNA含量，分析其DNA倍体类型。术后随访（9个月～32个月），调查病人复发或转移情况并计算生存期。结果28例原发性肝癌中16例（57．1％）为异倍体癌细胞、11例（39．3％）为二倍体癌细胞、1例（3．6％）为s期比值（SPF）〉19．6％、G2期比值〈15．0％的肝癌细胞。16例异倍体肝癌病人中，62．5％（10／16）的病人达不到10个月内无瘤生存（复发），中位无瘤期为4．3个月；1例病人DNA指数（DI值）为1.0、SPF〉19．6％、G2期比值〈15．0％的肝癌，术后7天即死亡；11例二倍体肝癌的复发率达9．1％（1／11），中位无瘤期为16．6个月，两者差异显著（x^2=9．68，P〈0．01）；16例异倍体肝癌病人的1年生存率为25．0％（4／16），2年生存率为6．3%（1／16）。中位生存期为9．5个月；11例二倍体肝癌病人的1年生存率达90．9％（10／11），2年生存率达63．6％（7／11），中位生存期为19．4个月，两者差异显著（1年生存率和2年生存率比较，x^2=18．766，P〈0．01；x^2=10．29，P〈0．01）。结论二倍体肝癌较异倍体肝癌的复发率低，无瘤生存期长，1年和2年生存率高，预后较好。可用肝癌细胞的DNA倍体类型评估肝癌病人的预后。Objective To find out the relation between the DNA ploidy of primary liver cancer and life-span of patients after operation. Method The DNA content of tumorous cell was detected by flow cytometry and the nuclear DNA ploidy was determined. The metastasis and life-span of patients were followed-up for 9 to 32 months after operation. Result Out of 28 patients with primary liver cancer, the tumorous cell DNA in 16 （57. 1%） was aneuploidy and 11 （39.3%） diploid. Another one （3.6%） was with S-phase faction（SPF） proportion 2〉19. 6% and G2 〈15%. In 16 patients with DNA aneuploidy, the recurrence rate in 10 months was 62. 5% （10/16）, and the median survival period was 4. 3 months ; one patient with S-phase faction died seven days after operation ; in 11 patients with diploid, the recurrence rate was 9.1%（1/11）, and the median survival period was 16.6 months（x^2 =9. 68,P〈0. 01） ;Survival rate in one year was 25. 0%（4/16） in 16 with aneuploidy, two years was 6.3%（1/16） ,and the median survival period was 9.5 months;Surviv al rate in one year was 90. 9%（10/11） in 11 with diploid , two years was 63.6%（7/11）, and the median survival period was 19. 4 months （x^2= 18. 76,P〈0.01 and x^2 = 10.29, P〈0. 01, respectively）. Conclusion On the contrary to aneuploidy, the relapse rate in diploid is much lower, the interval of neoplasia-free living is longer , and the survival rate in one year and two year are higher. So the DNA ploidy of primary liver cancer can help assess the prognosis of patient.

关 键 词：原发性肝癌 DNA倍体类型 预后 

分 类 号：R735.7[医药卫生—肿瘤]