HPL0C-MS/MS联用技术定量测定人血浆中奥洛他定浓度  

Quantitative Determination of Olopatadine in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：梁伟[1] 周辉[1] 刘东阳[1] 胡蓓[1] 江骥[1] 

机构地区：[1]中国协和医科大学北京协和医院临床药理中心,北京100730

出　　处：《质谱学报》2006年第4期193-197,共5页Journal of Chinese Mass Spectrometry Society

摘　　要：建立HPLC-MS／MS方法定量测定人血浆中奥洛他定浓度。血浆样品在酸性条件下，经Tomtec全自动固相萃取仪处理后．以v（甲醇）：v（0．05％甲酸）=50：50溶液为流动相，经AlltimaC18色谱柱初步分离，利用串联质谱在正离子电离模式下，以多反应离子监测（MRM）方式定量测定奥洛他定及其内标。结果表明：奥洛他定的最低定量浓度为0．1ng／mL，线性范围为0．1～100ng／mL．精密度和准确度均在±15％以内。此方法专属、灵敏、准确，可以用于测定人血浆中奥洛他定浓度。A sensitive and specific HPLC-MS/MS method was developed for the quantitative determination of olopatadine in human plasma. Plasma samples were prepared by Tomtec automatic SPE device and separated on an Alltima C18 column with methanol ： 0.05 % formic acid----50 ： 50 （v/v） as the mobile phase. Detection was performed on a triple-quadrupole tandem mass spectrometer using positive electrospray ionizatiofi （ESI）, and multiple reaction monitoring （MRM） was applied. The results showed the lowest limit of quantitation of olopatadine was 0. 1 ng/mL, and the linear range was 0.1～100 ng/mL. Accuracies and precisions of all were within ± 15%. This method proved to be specific, sensitive and accurately, which can be applied to determine olopatadine in human plasma.

关 键 词：奥洛他定 定量测定 血浆 HPLC—MS/MS 全自动固相萃取 

分 类 号：O657.63[理学—分析化学]