基于细胞色素b基因序列对蜻科部分种类系统进化研究(蜻蜓目:差翅亚目)  

Phylogeny of Libellulidae Based on Mitochondrial Cytochrome b Nucleotide Sequences (Odonata∶Anisoptera)

作　　者：张大治[1] 戴金霞[1] 郑哲民[2] 

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]陕西师范大学生命科学学院

出　　处：《四川动物》2006年第4期695-699,共5页Sichuan Journal of Zoology

基　　金：宁夏大学自然科学基金资助项目(NS0511)

摘　　要：采用PCR产物直接测序法,获得了蜻科6属9种蜻蜓的线粒体细胞色素b(Cytb)核苷酸序列(长度576 bp)。A+T平均含量较高,为69.2%。氨基酸密码子第三位点的A+T平均含量最高(86.5%)。变异位点数为216个(约占37.5%),简约信息位点148个,数据显示这几种蜻蜓之间DNA序列变异丰富。用一种豆娘Megalestes maai作外群构建系统发育树表明:蜻科为单系群,灰蜻属较进化,黄蜻属相对较古老一些。这6属的起源关系为:黄蜻属和赤蜻属→宽腹蜻属→椎腹蜻属和红蜻属→灰蜻属。In this study, fragments of mitochondrial DNA cytochrome b gene （each 576 base pairs in length） have been characterized from twenty individuals belong to 9 species from 6 genera of Libellulidae in China. These sequences were parts of Cyt b gene of mtDNA, and in the obtained sequences, A% ＋ T% was about 69.2%. From every amino acid codon, A% ＋ T % in the third site was higher （86.5 % ） than the other two sites. The sequence data revealed considerable variation in 216 nucleotide sites （about 37.5 % ） among the analyzed individuals from 6 genera. We obtained homologous nuclear sequence of Megalestes maai （damsdfly） for outgroup. The phylogenetic trees were constructed with neighbor-joining method, maximum likelihood method and maximum parsimony method. The confidence of nodes in the trees was evaluated by 1000 replicates bootstrap. The phylogenetic relationship of mitochondrial DNA suggested that the Libellulidae was a monophyletic group. Orthetrum was more evolutional than other genera. The phylogenetic relationships of the 6 genera was： Paniala and Sympelrum→ Lyriothemis→ Acisoma and Crocothemis →Orthelrum .

关 键 词：细胞色素b(Cyt b) 线粒体DNA(mtDNA) 系统进化 蜻科 蜻蜓目 

分 类 号：Q961[生物学—昆虫学] Q969.22