红景天对大鼠小肠缺血再灌注损伤的保护作用  

Protective effects of Hongjingtian on intestinal ischemia/reperfusion injury in rats

作　　者：赵正维[1] 王为忠[1] 陈宏[2] 于小迪[1] 

机构地区：[1]解放军第四军医大学西京医院胃肠外科,陕西省西安市710033 [2]解放军第四军医大学西京医院中医药研究中心,陕西省西安市710033

出　　处：《中国临床康复》2006年第43期117-119,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:利用SD大鼠制备肠缺血再灌注模型,观察红景天对肠缺血再灌注损伤的保护作用及其作用机制。方法:实验于2005-08/2006-05在第四军医大学西京医院胃肠外科实验室完成。雄性SD大鼠30只,体质量200～250g,随机数字表法分为假手术组、缺血再灌注组、红景天处理组,每组10只。红景天液制法参照中药质量标准:取红景天生药102g,加水煎煮2次,第1次1.5h,第2次1h,滤过,滤液合并后加水调至120mL,每只大鼠2mL/d,相当于生药5g/(kg·d)。缺血再灌注模型制备:动物于术前12h禁食,不禁水。以2%盐酸氯胺酮(100mg/kg)腹腔注射麻醉。生理盐水2mL/d,连续灌胃5d后,取正中切口入腹后分离肠系膜上动脉,以无创伤血管夹阻断肠系膜上动脉根部造成小肠完全缺血60min,然后松夹进行再灌注60min。假手术组:生理盐水2mL/d,连续灌胃5d后,只分离肠系膜上动脉,但不作阻断。红景天处理组:红景天按5g/kg生药,溶于2mL水中灌胃给药,连续5d后,分离肠系膜上动脉,夹闭肠系膜上动脉60min后再灌注60min。检测各组大鼠小肠黏膜中肿瘤坏死因子α、白细胞介素-6水平,以及其中的超氧化物歧化酶和丙二醛的含量。结果:30只大鼠均进入结果分析。①缺血再灌注组肿瘤坏死因子α、白细胞介素-6水平均高于假手术组和红景天处理组[肿瘤坏死因子α:(2.57±0.29),(0.32±0.06),(1.06±0.11)μg/L;白细胞介素-6:(965.73±42.01),(122.46±1.74),(385.03±13.50)ng/L,P<0.01]。②缺血再灌注组丙二醛水平高于假手术组和红景天处理组[(0.68±0.11),(0.33±0.10),(0.54±0.12)nmol/g,P<0.01];超氧化物歧化酶水平低于假手术组和红景天处理组[(34.12±5.31),(92.63±3.82),(55.66±5.92)NU/g,P<0.01]。结论:红景天可通过抑制细胞因子表达及清除氧自由基的方式,对肠缺血再灌注损伤起到保护作用。AIM： To investigate the protective effect of Hongjingtian on intestinal ischemia/reperfusion （I/R） injury in SD rat and the possible mechanism. METHODS： The experiment was conducted in the laboratory of Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA from August 2005 to May 2006, A total of 30 male SD rats with the body mass of 200-250 g were randomly divided into three experimental groups （n=10 in each）： sham operation group, ischemia/reperfusion （I/R） group and Hongjingtian treated I/R group with 10 in each group. Method of preparation of Hongjingtian liquid was accorded with the quality criteria of traditional Chinese medicine： Hongjingtian crude drug of 102 g were decocted with water twice, 1.5 hours at the first time and 1 hour at the second time, filtered, water was added after filtrate complicating till 120 mL, 2 mL every day in each rat, which was equal to 5 g/kg crude drug per day. I/R model preparation： animals were fasting 12 hours before operation, but water was given. 100 mg/kg 2% ketamine hydrochloride was treated intraperitoneally. After 2 mL/d saline by gastric perfusion successively for 5 days, superior mesenteric artery was separated by median incision, Ischemia in small intestine was made by blocking the superior mesenteric artery with vascular clamp for 60 minutes, and then the clamp was loosed to make reperfusion for 60 minutes. Sham operation group： 2 mL/d saline was given successively by gastric perfusion for 5 days. Only superior mesenteric artery was separated, but no block. Hongjingtian treated I/R group： 5 g/kg Hongjingtian crude drug dissolved in 2 mL water was given by gastric perfusion for 5 days successively, and then superior mesenteric artery was separated. The superior mesenteric artery was occluded for 60 minutes, and then re-perfused for 60 minutes. The levels of tumor necrosis factor alpha （TNF-α） and interleukin 6 （IL-6） and the contents of superoxide dismutase （SOD） and malond

关 键 词：小肠 缺血/病理学 再灌注 红景犬/治疗应用 

分 类 号：R574.5[医药卫生—消化系统]