PCR对弓形虫感染家兔血液中虫体DNA的动态检测  

A dynamic study on Toxoplasma gondii DNA in blood from infected rabbits by PCR

作　　者：秦川[1] 刘世国[2] 姚志军[2] 杨献军[1] 

机构地区：[1]新乡医学院分子生物学研究室,河南新乡453003 [2]新乡医学院寄生虫学教研室,河南新乡453003

出　　处：《新乡医学院学报》2006年第6期559-560,共2页Journal of Xinxiang Medical University

基　　金：河南省高校杰出人才创新工程项目(编号:2006KYCX011)

摘　　要：目的了解弓形虫感染家兔血液中是否有虫体存在，探明虫体在血液中动态变化情况。方法用不同剂量的RH株弓形虫速殖子经腹腔感染家兔6只。在家兔感染前后分别采集血液，抗凝处理后，-40℃冻存备用，用普通PCR检测弓形虫感染家兔血液中弓形虫DNA。结果5只家兔在感染后的6～14d死亡，仅1只家兔在感染后的6-72d，用PCR检测手段在血液中均可检测到弓形虫DNA。结论弓形虫感染雄兔血液中有虫体存在。Objective To assess the presence and dynamic changes of Toxoplasma gondii DNA in blood from infected rabbits. Methods The blood from 6 infected rabbits were sampled at one week interval before and after infection,and freezed at -40℃ after anticoagulation treatment. The Toxoplasma gondii DNA were detected by polymerase chain reaction （PCR） from rabbit samples. Results Five rabbits died from parasite infection at day 6-- 14 post - infection. The Toxoplasma gondii DNA can be detected in blood samples from only one survival infected rabbit at day 6 and 72 post - infection. Conclusion It is present in blood the Toxoplasma gondii DNA from the infected rabbits.

关 键 词：弓形虫 血液 聚合酶链反应 家兔 

分 类 号：R382.5[医药卫生—医学寄生虫学]