树突状细胞融合瘤苗抗胶质瘤的实验研究  

The experimental study of antitumor immunity of fusion vaccine of dendritic cell

作　　者：王东海[1] 李新钢[1] 曲迅[2] 李刚[1] 宫崧峰[1] 黄齐兵[1] 刘泉萌[1] 鲍修风[1] 

机构地区：[1]山东大学齐鲁医院神经外科,250012 [2]山东大学齐鲁医院临床实验中心

出　　处：《中华神经外科杂志》2006年第11期699-701,共3页Chinese Journal of Neurosurgery

基　　金：山东省科技厅资助项目(2000BB1CJA13)

摘　　要：目的观察树突状细胞(dendritic cells,DC)融合瘤苗防治C6胶质瘤的疗效,对其抗肿瘤作用机制进行初步探讨。方法采用PEG化学融合方法制备融合瘤苗,流式细胞技术分选瘤苗,对融合瘤苗进行免疫组化和功能学鉴定；立体定向制备大鼠颅内C6肿瘤模型,荷瘤后5d经尾静脉注射10~7融合瘤苗、10~7DC以及100μl PBS,分别设为A、B、C三组,采用Log-rank对数秩检验进行生存分析,对肿瘤标本进行病理学检查。结果实验中DC具有典型的树突状结构,OX_(62)特异性标志物阳性表达；融合瘤苗GFAP-FITC免疫荧光检查阳性；Log-rank生存分析数据对数秩检验表明A组与B、C组比较均有统计学意义(P＜0．01)；A组晚期死亡大鼠(＞31d)HE染色见较多的炎性细胞浸润,CD_8^+ Mcab免疫组化染色阳性。结论PEG法可有效制备DC/C6融合瘤苗,融合瘤苗能够有效的发挥抗肿瘤效果,CD8^+ T细胞参与颅内抗胶质瘤免疫反应。Objective To study the anti-tumor effect and mechanism of fusion vaccine of DCs and C6 glioma cells. Methods PEG additive was used to fuse DCs with C6 glioma cells. Fusion cells were selected by flow cytometry . Identification of DC/C6 fusion cells was conducted by immunofluorescence with GFAP-FITC. Rat brain glioma models were established by stereotactic technique. After 5 days of inoculation, 107 fusion cells were injected through tail vein in group A. The same mumber of DCs and the same volume of PBS were applied to group B and group C. The survival time of rats in these three groups was analyzed by Log-rank survival analysis. Tumor samples were examined with HE staining and immunohistochemistry with CDs Mcab. Results At day 10, DC appeared typical long dendrite in morphology and expressed special OX62 marker. Immunofluorescence with GFAP-FITC showed positive in DC/C6 fusion cells. The Log-rank survival analysis showed group A was statistically significant in contrast to group A and group B （P 〈 0.01 ）. Tumor samples in group A stained with HE showed many inflammation cells infiltrated and the immunohistochemistry with CDs Mcab staining was Positive. Conclusion PEG chemical fusion method is an effective way to prepare fusion vaccine. DC/C6 fusion cells have the ability of activating antitumor immunity. CD8^＋ T lymphocyte take an important role in antitumor immunity.

关 键 词：树突细胞 神经胶质瘤 融合瘤苗 免疫疗法 

分 类 号：R739.45[医药卫生—肿瘤]