基质金属蛋白酶1/组织金属蛋白酶抑制因子1在常染色体显性遗传性多囊肾组织中的表达  

Expression of matrix metalloproteinases-1/tissue inhibitor of metalloproteinase-1 in kidney of patients with autosomal dominant polycystic kidney disease

作　　者：崔心刚[1] 安瑞华[2] 王立明[3] 朱有华[3] 梅长林 

机构地区：[1]第二军医大学长征医院泌尿外科解放军泌尿外科中心,上海200003 [2]哈尔滨医科大学附属第一医院泌尿外科,哈尔滨150001 [3]第二军医大学长征医院器官移植科解放军器官移植研究所,上海200003 [4]长征医院肾内科,解放军肾脏病研究所

出　　处：《第二军医大学学报》2006年第11期1174-1177,共4页Academic Journal of Second Military Medical University

基　　金：黑龙江省博士后基金(LRB04-228);第二军医大学长征医院"三重三优"优秀青年后备人才基金~~

摘　　要：目的:探讨基质金属蛋白酶1(MMP1)/组织金属蛋白酶抑制因子(TIMP1)在正常肾脏、常染色体显性遗传性多囊肾病(ADPKD)肾脏以及肾移植平均(2.5±1.2)年后切除的ADPKD肾组织中的表达差异。方法:用基因表达谱芯片筛选正常肾脏、ADPKD肾脏及肾脏移植后ADPKD肾脏的差异表达基因;以RT-PCR法验证其中差异表达的MMP1/TIMP1。结果:基因芯片筛选发现在正常肾组织与ADPKD肾组织中存在463条差异表达基因,肾移植后ADPKD肾组织对比ADPKD肾组织存在130条差异表达基因。筛选结果表明ADPKD以及肾移植后ADPKD肾脏MMP1/TIMP1表达较正常肾组织明显上调(P<0.05),而前两者间无显著差异。RT-PCR法证实MMP1/TIMP1在ADPKD肾脏以及肾移植后ADPKD肾组织中的表达均明显上调,明显高于正常肾脏组织(P<0.05),而前两者间无显著差异。结论:ADPKD的病理改变可能与MMPs/TIMPs基因表达失衡有关,MMPs抑制剂可能会对其有一定的治疗作用。Objective： To investigate the differential expression of matrix metalloproteinases-1 / tissue inhibitor of metalloproteinase-1 （MMP-1/TIMP-1） between normal kidney,kidneys of patients with autosomal dominant polycystic kidney disease （ADPKD）, and the original kidneys after renal transplantation（OKRT）. Methods： DNA microarray technique was used to analyze the differential gene expression in the above 3 tissues. Semi-quantitive RT-PCR was performed to verify the differentially expressed genes. Results： There were 463 differentially expressed genes between normal kidney and ADPKD tissues and 130 differentially expressed genes between ADPKD and the OKRT tissues. Expression of MMP1/TIMP1 in the ADPKD and the OKRT tissues were significantly higher than that in the normal kidney tissue （P〈0.05）, with no significant difference found between the former 2 groups. Results of RT-PCR were consistent with the microarray findings. Conclusion： The pathogenesis of ADPKD may be related with the high expression of MMPs/TIMPs and the inhibitor of MMPs may have therapeutic effect on ADPKD.

关 键 词：基质金属蛋白酶1 组织金属蛋白酶抑制因子1 肾 肾移植 多囊肾 常染色体显性 

分 类 号：R692[医药卫生—泌尿科学]