动脉粥样硬化小型猪SR-BI和PPARγ表达的变化  

CHANGES OF SCAVENGER RECEPTOR CLASS B TYPE I AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR γ EXPRESSION IN ATHEROSCLEROTIC MINI SWINE

作　　者：易光辉[1] 莫中成[2] 曾颖[1] 尹小波[1] 刘录山[1] 王佐[1] 冯惊涛[1] 曾德星[1] 孙琳[1] 

机构地区：[1]南华大学心血管病研究所,湖南衡阳421001 [2]南华大学组织胚胎学教研室,湖南衡阳421001

出　　处：《中国应用生理学杂志》2006年第4期439-443,I0002,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30570958);江苏省"135工程"开放课题资助项目

摘　　要：目的:用贵州小香猪建立动脉粥样硬化(As)动物模型,探讨该模型中B类I型清道夫受体(SR-BI)和过氧化物酶体增殖物激活受体γ(PPARγ)表达的变化。方法:采用血管内膜损伤法加高脂高胆固醇饲料喂养贵州小香猪,氧化酶法测定血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)的浓度。12个月后处死动物,采用苏丹Ⅳ及HE染色检测肝脏脂质沉积及动脉粥样斑块病变;逆转录-聚合酶链反应(RT-PCR)、Westernblot印迹和免疫组织化学法分别检测肝、主动脉组织中SR-BI、PPARγ的mRNA和蛋白质表达。结果:实验组与对照组比较,血浆TC、TG、HDL-C水平升高;实验组小型猪肝脏有大量的脂肪空泡,动脉有明显的脂质条纹及斑块;动脉粥样硬化小型猪肝组织、主动脉组织的SR-BI、PPARγ的mRNA及蛋白质的表达均上调(P<0.05)。结论:本实验说明颈总动脉内膜拉伤加高脂高胆固醇饲料喂养小型猪可建立As动物模型;As小型猪肝组织、主动脉组织的SR-BI和PPARγ表达上调。To study the expressions of scavenger receptor class B type Ⅰ （SR-BI） and peroxisome proliferator-activated receptor γ （PPARγ） in atherosclerotic mini swine and provide a new mechanism for investigating the pathogenesis of atherosclerosis. Methods： Chinese mini swine were fed by a normal control diet or a high fat/high cholesterol diet for 12 months after common carotid artery injury induced by balloon denudation. Plasma total cholesterol （TC）, high-density lipoprotein cholesterol（HI）L-C） and triglycerides （TG） were determined by commercially enzymatic methods every two months. The sections, which were taken from liver and abdominal aorta, were stained with hematoxylin eosin. The expressions of SR-BI and PPARγ mRNA and protein in liver and aorta tissue were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）, Western blot and immunohistochemistry respectively. Results： At the end of 12 months, plasma TC, HDL-C and TG in HFHC mini swine were increased. There were fatty liver and atherosclerotic plaque in mini swine live and aorta respectively. The expression of SR-BI was upregulated in HFHC mini swine liver and aorta tissue.Conclusion： HFHC may induce atherosclerosis and the expression of SR-BI and PPARγ. Upregulating SR-BI expression may inhibit atherosclerosis. Increasing SR-BI expression in liver and aorta may accelerate SR-BI-mediated reverse cholesterol transport and develop a new anti-atherogenic strategy.

关 键 词：动脉粥样硬化 B类Ⅰ型清道夫受体 过氧化物酶体增殖物激活受体Γ 小型猪 

分 类 号：R363[医药卫生—病理学]