重组人Ⅱ型胶原250-270多肽对特异性体液免疫的抑制作用  

Study of the humoral immune suppression of recombinant human collagen type Ⅱ peptide 250-270

作　　者：王宏坤[1] 朱平[1] 李晓燕[1] 郑朝晖[1] 贾俊峰[1] 丁进[1] 樊春梅[1] 

机构地区：[1]第四军医大学西京医院临床免疫科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2006年第6期742-744,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重点科技攻关项目基金资助(9690105262)

摘　　要：目的:研究口服重组人Ⅱ型胶原250-270多肽(CⅡ250-270)对胶原诱导性关节炎(CIA)小鼠特异性体液免疫反应的抑制作用,为应用口服CⅡ250-270治疗类风湿关节炎提供理论依据。方法:ELISA测定加强免疫后小鼠血清中抗原特异性抗体的表达,ELISPOT检测小鼠脾脏淋巴细胞中抗原特异性抗体形成细胞。结果:口服重组人CⅡ250-270小鼠血清中抗CⅡ和抗CⅡ250-270的IgG抗体水平[A值分别为(0.82±0.02)、(0.84±0.04)]比CⅡ免疫对照组[抗CⅡ的IgG抗体水平,A值为(1.01±0.06)]显著降低,而且特异性抗CⅡ250-270的IgG抗体反应在口服多肽组被明显抑制(P<0.01);口服重组人CⅡ250-270小鼠的CⅡ和CⅡ250-270特异性抗体形成脾细胞的增殖频率分别为(158±9计数/孔)、(181±10计数/孔),比CⅡ免疫对照组[CⅡ特异性抗体形成脾细胞的增殖频率为(247±16计数/孔)]也显著减少(P<0.05)。结论:口服重组人CⅡ250-270多肽具有抑制CIA小鼠的特异性体液免疫的作用,可能是此疗法治疗CIA多发性关节炎的重要机制。AIM： To investigate the modulation of rhC Ⅱ 2.50-270 peptide on special humoral immune response in the course of oral administration. METHODS： ELISA was used to determine antigen-specific antibodies in mice sera. The frequency of anti-C Ⅱ and anti-C Ⅱ （2.50-270） antibody-forming spleen cells was measured by ELISPOT. RESULTS： The level of C Ⅱ - and C Ⅱ （2.50-270）- specific IgG in serum from the mice fed with rhCⅡ（2.50-270） were （0.82 ±0.02）and （0. 84 ± 0. 04 ） respectively, and lower significantly than those of collagen-induced arthritis（CIA） control group. The anti-C Ⅱ （2-50-270） antibody responses were suppressed obviously （ P 〈 0.01 ）. The frequency of antibody-forming cells in the spleen from rhC Ⅱ （2.50-270）-fed mice were （ 1.58 ± 9 counts/well） and （ 181 ± 10 counts/well） respectively, and also were reduced significantly when compared with that in CIA control group （247 ± 16 counts/well） （ P 〈 0.0.5 ）. CONCLUSION： Oral rhC II （2.50-270） could induce specific suppression of humoral responds in CIA. These findings together with a better understanding of the mechanisms of oral tolerance and regulation of humoral immune response in CIA, will help the development of innovative therapeutic intervention for rheumatoid arthritis.

关 键 词：类风湿关节炎 胶原诱导性关节炎 Ⅱ型胶原 Ⅱ型胶原250—270多肽 口服耐受 

分 类 号：R593.2[医药卫生—内科学]