B-ALL患者胸腺输出TCR Vβ亚家族nave T细胞分析  

Analysis of Thymic Output of TCRVβ Subfamily nave T Cells in Patients with B-ALL

作　　者：李闯[1] 李扬秋[1] 尹青松[1] 陈少华[1] 杨力建[1] 胡刚[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632

出　　处：《肿瘤防治研究》2006年第11期788-790,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(30270579);广东省自然科学基金重点项目(23001);广东省教育厅"千百十工程"优秀人才培养基金项目(Q校02022);血液病学国务院侨办重点专科基金资助

摘　　要：目的了解B细胞-急性淋巴细胞白血病（B-ALL）患者外周血T细胞TCR Vβ亚家族23种信号T细胞受体删除DNA环（sjTRECs）的存在情况，从而了解胸腺近期输出各TCR Vβ亚家族naive T细胞的功能。方法利用半巢式PCR分别扩增10例B-ALL患者外周血单个核细胞DNA中的23种Vβ～Dβ1 sjTRECs，10例正常人作为对照。结果TCR Vβ亚家族sjTRECs在大部分正常人和部分B-ALL患者中均可检测到，与正常人比较，B-ALL病人的Vβ24～Dβ1 sjTRECs出现频率比正常人高，且有统计学意义（80％：30％，P=0．0285），另有18个家族的Vβ～Dβ1 sjTRECs比正常人低，其中在Vβ1（30％，P=0．0285）、Vβ12（20％，P＝0．0285）、Vβ13（40％，P=0．0043）、Vβ15（20％，P=0．0022）和Vβ18（20％，P=0．0089）5个亚家族sjTRECs出现频率的差异有统计学意义。结论B-ALL病人胸腺输出大多数Vβ亚家族naive T细胞功能低于正常人。Objective In order to investigate the recent thymic output function of TCR Vβ subfamily naive T cells in patients with B cell-acute lymphoblastic leukemia （B-ALL） by detection of the frequencies of 23 subfamilies of TCR Vβ sjTRECs in peripheral blood T cells. Methods 23 subfamilies of Vβ-Dβ1 sjTRECs were amplified in genomic DNA of peripheral blood mononuclear cells （PBMC） from 10 cases with B- ALL by using semi-nest PCR. 10 normal individuals served as controls. Results TCR Vβ subfamilies of sjTRECs were detected in normal individuals and some cases with B-ALL. Compared with the normal control, the frequencies of Vβ24-Dβ1 sjTRECs in B-ALL patients （80%） was significant higher than in the normal individuals （30%, P = 0. 0285）, while lower frequencies were found in the other 18 Vβ subfamily sjTRECs in B-ALL. The frequencies of Vβ1 （30%, P = 0. 0285）, Vβ12-（20%, P = 0. 0285）, Vβ13-（40%, P = 0. 0043）, Vβ15-（205, P = 0. 0022） and Vβ18-Dβ1 sjTRECs （20%, P = 0. 0089） subfamilies were significant lower than in the normal individuals. Conclusion The recent thymic output function of most Vβ subfamily naive T cells in patients with B-ALL was lower than in the normal individuals.

关 键 词：急性淋巴细胞白血病 T细胞受体删除DNA环 胸腺近期输出功能 

分 类 号：R733.73[医药卫生—肿瘤] R371.22[医药卫生—临床医学]