流式细胞术对食管癌、贲门癌患者T细胞亚群的分析  

The Analysis of T Lymphocyte Subsets Using FCM to the Peripheral Blood of Patients with Esophageal or Cardiac Carcinoma

作　　者：沈玉光[1] 高宗人[1] 许金良[1] 杨宗林[2] 赵婷茹[3] 

机构地区：[1]河南省肿瘤医院胸外科,郑州450008 [2]四川省广元市剑阁县人民医院胸外科 [3]河南省肿瘤医院病理科

出　　处：《肿瘤防治研究》2006年第11期818-820,共3页Cancer Research on Prevention and Treatment

摘　　要：目的采用流式细胞术方法检测食管癌、贲门癌患者细胞免疫功能。方法应用三色免疫荧光流式细胞仪测定145名食管癌和贲门癌患者及18例正常对照者外周血T细胞亚群CD3+、CD4+、CD8+、NK细胞、CD4+/CD8+比值。结合临床病理结果探讨二者之间的关系。结果(1)食管癌、贲门癌患者外周血中CD8+T淋巴细胞比例较正常对照组明显升高(P=0.030);CD3+、CD4+亚群较正常值降低,但是没有统计学差异。NK细胞两组比较也没有显著性差异;(2)早期(0、Ⅰ期)食管癌、贲门癌病人和健康对照组之间T细胞各亚群相比没有统计学差异;中、晚期(Ⅰ、Ⅱ、Ⅲ期)食管癌、贲门癌病人和健康对照组相比CD4+明显降低(P=0.023);CD8+则明显升高(P=0.021);CD4+/CD8+较正常有所下降,但是没有显著性差异;淋巴结阴性患者和淋巴结阳性患者比较T细胞各亚群没有明显差异。结论外周血T细胞亚群的检测有助于提高对食管癌患者的病情,临床分期以及机体免疫状态的判断,晚期食管癌患者机体免疫状态成紊乱型改变。Objective To measure the state of cellular immunity of patients with esophageal or cardiac carcinoma by flow cytometry. Methods Venous blood was obtained from 145 patients with esophageal or cardiac carcinoma and 18 healthy volunteers. Percentages of CD3^＋ , CD4^＋ , CD8^＋ , NK cell and CD4^＋ / CD8^＋ ratio were measured by flow cytometry, and explored the relationship between clinical stage and T lymphocyte subproportion. Results Compared to controls, 145 patients with esophageal or cardiac carcinoma had significant higher percentages of CD8^＋ T cells. No differences in the percentages of CD3^＋ , CD4^＋ T cells and the CD4^＋/CD8^＋ ratio between patients and normal controls were observed. Although there were not differences in the percentages of CD3^＋ , CD4^＋ and CD8^＋ T cells between the stage 0 and Ⅰ and the controls, However, the patients who had advanced carcinoma（stage Ⅱ , Ⅲ, Ⅳ, ） had significant higher percentages of CD8^＋ T cells and lower CD4^＋ T cells than that of the controls. The tumor-node metastasis （TNM） stage and lymphocyte node involvement was not related to the T-cell subsets. Conclusion Analyses of T Lymphocyte subset can provide useful information on the function of the patients with esophageal or cardiac carcinoma.

关 键 词：食管癌 贲门癌 T细胞亚群 流式细胞术 临床分期 

分 类 号：R735.1[医药卫生—肿瘤]