人肺腺癌细胞SPC-A1对氨基硅烷纳米Fe_3O_4内吞途径及机制的初步研究  

Internalization pathway of aminosilane-coated Fe_3O_4 nanoparticles by human lung adenocarcinoma SPC-A1 cells

作　　者：马勇杰[1] 鄢祝兵[1] 古宏晨[1] 

机构地区：[1]上海交通大学微米/纳米加工技术国家重点实验室薄膜与微细技术教育部重点实验室,上海200032

出　　处：《肿瘤》2006年第11期967-969,共3页Tumor

基　　金：国家863基金(编号:2004AA302023;2005AA3021110)

摘　　要：目的：探讨氨基硅烷纳米Fe_3O_4进入到人肺腺癌细胞SPC-A1的内吞途径及其机制。方法：在不同温度及细胞松弛素B处理前后对人肺腺癌细胞SPC-A1进行培养,通过透射电镜动态观察细胞对氨基硅烷纳米Fe_3O_4的不同内吞情况。结果：氨基硅烷纳米Fe_3O_4颗粒与SPC-A1细胞在低温条件混合培养时可阻断氨基硅烷纳米Fe_3O_4颗粒进入细胞质,而以静电作用的方式吸附于SPC-A1细胞膜表面。然而,当处于37℃条件培养时可激活细胞内吞作用,纳米颗粒首先进入细胞表面包被小窝、通过内吞体进入溶酶体。但经采用细胞松弛素B处理后,可导致37℃培养的SPC-A1细胞无法内吞纳米颗粒。结论：氨基硅烷纳米Fe_3O_4系通过细胞吞噬途径而进入细胞质,Fe_3O_4纳米颗粒有可能作为一种研究细胞内吞的示踪剂。Objeetive：To study the internalization pathway of aminosilane-coated Fe3O4 nanoparticles by human lung adenocarcinoma SPC-A1 cells. Methods.. Human lung adenocarcinoma SPC-A1 cells were cultured at different temperatures or at 37℃ with or without cytochalasin B treatment. The dynamic endocytosis of aminosilane-coated Fe3O4 nanoparticles was observed by transmission electron microscopy. Results.. Low temperature could block the endocytosis of aminosilane-coated Fe3O4 nanoparticles into SPC-A1 cells. The nanoparticles were adsorbed on the plasma membrane probably via electrostatic interactions. When SPC-A1 cells were cultured at 37℃, the process of endocytosis was activated. Nanoparticles were taken up by the coated-pit on cell membrane. And then they were transported into endosomes. Finally they were localized in lysosomes. Endocytosis was inhibited after cytochalasin B treatment at 37℃. Conclusion.. Aminosilane-coated Fe3 04 nanoparticles were internalized into human lung adenocarcinoma SPC-A1 cells via endocytosis pathway. It could be used as a magnetic tracer for studying cell internalization.

关 键 词：肺肿瘤 吞噬作用 细胞内吞作用 三价铁化合物-氧化铁 纳米粒子 SPC-A1细胞 

分 类 号：R734.2[医药卫生—肿瘤] R73-33[医药卫生—临床医学]