TSA对肺腺癌细胞株A549细胞凋亡的影响  

Effect of trichostatin A on apoptosis of lung epithelial carcinoma A549 cells

作　　者：姜淑娟[1] 尚建强[1] 张嵩[1] 杨艳娜[1] 贾龏婷 

机构地区：[1]山东省立医院呼吸科,济南250021

出　　处：《肿瘤》2006年第11期1007-1010,共4页Tumor

摘　　要：目的：探讨组蛋白脱乙酰化酶抑制剂曲古抑菌素A(trichostatin A,TSA)对A549肺腺癌细胞凋亡的影响。方法：Annexin V和Hoechst染色法检测细胞凋亡；流式细胞仪分析细胞周期；Western blot检测凋亡信号通路中caspase-8、caspase-9活化及多聚ADP核糖聚合酶(PARP)裂解情况。结果：TSA可诱导细胞凋亡,主要使细胞积聚在G_2/M期,且呈浓度依赖性。Western blot检测表明TSA诱导了A549肺腺癌细胞caspase-8、caspase-9裂解活化及PARP裂解,且随TSA作用时间延长而逐步升高。结论：TSA诱导A549细胞凋亡中caspase-8、caspase-9介导caspase-3的活化,TSA通过caspase级联反应诱导A549细胞凋亡。Objective：To study the effect of histone deacetylase inhibitor trichostatin A （TSA） on apoptosis of lung epithelial carcinoma A549 cell line. Methods：Apoptosis was observed by Annexin V and Hoechst staining. The cell cycle was analyzed by flow cytometry. Activation of caspase-8 and caspase-9 and cleavage of poly （ADP-ribose） polymerase （PARP） were detected by Western blot. Results：TSA induced apoptosis of A549 cells and arrested cells in G2/M phase in a dose-dependent manner, Western blot analysis showed that TSA induced activation of caspase-8 and caspase-9 and cleavage of PPAR of A549 cells in a time-dependent manner. Conclusions： TSA induced caspase-8 and caspase-9-mediated caspase-3 activation during apoptosis of A549 cells. TSA induces the apoptosis of A549 cells via caspase cascade pathway.

关 键 词：肺肿瘤 曲古抑菌素A 细胞凋亡 A549细胞 

分 类 号：R734.3[医药卫生—肿瘤]