Syk在不同组织来源的人腺上皮恶性肿瘤中的表达  

Expression of Syk in human glandular epithelium malignant tumor tissues from different origins

作　　者：史健[1] 单保恩[1] 周艳[1] 宋小珍[1] 丁春艳[1] 鲁壮平[1] 杜恩起[1] 

机构地区：[1]河北医科大学第四医院肿瘤内科,石家庄050011

出　　处：《肿瘤》2006年第11期1028-1032,共5页Tumor

基　　金：河北省科技厅科技攻关项目(编号:05276101D-59)

摘　　要：目的：探讨不同组织来源的人腺上皮恶性肿瘤细胞中Syk的表达,分析其临床意义。方法：应用免疫组织化学染色法分别检测7种人腺上皮恶性肿瘤细胞株、30例人乳腺癌组织及其35例人胰腺癌组织中Syk的表达。结果：人乳腺癌细胞株MCF-7中Syk表达阳性,MDA-MB-231则表达阴性；人胰腺癌细胞株PANC-1、SW1990和Canpan-2,人肺腺癌细胞株GLC-82和肝癌细胞株SMMC-7721中Syk均表达阳性。30例乳腺癌组织及其癌旁正常组织中Syk表达阳性率分别为60% (18/30)和90%(27/30),两者之间有显著性差异(P＜0．01)。22例胰腺癌组织及其癌旁正常组织中Syk表达阳性率分别54．5%(12/22)和86．3%(19/22),两者之间有显著性差异(P＜0．01)；13例经术前化疗的胰腺癌组织及其癌旁正常组织中Syk表达阳性率分别为69．2%(9/13)和92．3%(12/13),两者之间有显著性差异(P＜0．01)。结论：Syk在所检测的不同组织来源的人腺上皮恶性肿瘤细胞株中绝大部分(6/7)呈阳性表达；Syk蛋白在乳腺、胰腺正常组织中高表达,在相应肿瘤组织中低表达或不表达的特性,Syk有可能成为乳腺癌患者的预后判定、治疗计划的制定等较为特异性指标。Objective：To study the expression of Syk in the human glandular epithelium malignant tumor tissues form different origins and analyze its clinical significance. Methods： Syk expression was detected by immunohistochemical staining in 7 human glandular epithelium malignant tumor cell lines, 30 specimens from human breast cancer tissues, and 35 specimens from human pancreatic cancer tissues. Results： In human breast cancer cell line the Syk expression was positive in MCF-7 cells but negative in MDA-MB-231 cells. Syk was positively expressed in human pancreatic cancer cell line PANC1, SW1990, and Canpan-21 and human lung adenocarcinoma cell line GLC-82 and human hepatocellular carcinoma cell line SMMC-7721. The positive rate of Syk was 60% （18/30） in 30 breast cancer tissues and 90% （27/30） in adjacent non-cancerous breast tissues. The difference was significant （P〈0.01）. The positive rate of Syk was 69.2% （9/13） in pancreatic cancer tissues from 17 patients who had received preoperative chemotherapy and 92.3% （12/13） in adjacent non-cancerous tissues. The difference was significant （P〈0.01）. Conclusion： Syk expression was positive in human glandular epithelium malignant tumor tissues from different origins. Syk had higher expression in normal tissues and lower or no expression in tumor tissues. Expression of Syk had negative correlation with clinical staging and could be used as a specific maker for selecting therapeutic regimen and evaluating prognosis of breast cancer patients.

关 键 词：肿瘤 腺性和上皮性 蛋白质酪氨酸激酶 免疫组织化学 预后 

分 类 号：R735.9[医药卫生—肿瘤]