大鼠脑梗死后室周带神经干细胞增殖和迁移  

Generation of neural stem cells in subventricular zone and migration to the ischemic lesion area after cerebral infarction in rats

作　　者：郭芮兵[1] 朱武生[1] 徐格林[1] 马敏敏[1] 李芸[1] 刘新峰[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)神经内科,江苏南京210002

出　　处：《医学研究生学报》2006年第11期984-986,I0007,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30470604)

摘　　要：目的:研究成年大鼠脑梗死后室周带神经干细胞的增殖及迁移。方法:制作大鼠脑梗死模型,将其分为脑梗死后3、5、7和14 d组,对照组为假手术组。采用免疫组化法动态检测大鼠脑内5-溴脱氧尿苷嘧啶(B rdU)、双皮质素(DCX)的表达。结果:与对照组相比,室周带B rdU阳性细胞在脑梗死后逐渐增加,并于7 d达高峰,14 d后开始下降,但仍高于正常水平。梗死后不同时间组室周带B rdU阳性细胞数均高于对照组(P<0.01)。DCX阳性细胞具有迁移细胞的形态学特征,并向损伤区迁移,于术后5 d迁移细胞的数量达高峰。结论:脑梗死可激活室周带神经干细胞原位增殖,并向损伤区迁移。Objective：To investigate the generation and migration of inherent neural stem cells in the adult rats with cerebral infarction. Methods： Rat cerebral infarction model was made and the dynamic expressions of bromodeoxyuridine （BrdU） and doublecortin （DCX） were determined by immunofluorescence staining. Results：Compared with the controls, the number of BrdU immunoreactive cells in the subventricular zone （SVZ） increased significantly （P 〈 0.01 ） after focal cerebral infarction. The expression of BrdU cells was peaked at day 7, it decreased at day 14 but remained at a level higher than that of the controls. DCX immunoreactive cells in the SVZ were morphologically migrating, they migrated to the ischemic lesion and the number was peaked at day 5 （P 〈 0.01 ）. Conclusion ： The results indicated that generation of inherent neural stem cells was in the SVZ and the neural stem cells migrated to the ischemic lesion area after ischemic stroke.

关 键 词：脑梗死 神经干细胞 增殖 细胞迁移 

分 类 号：R743.3[医药卫生—神经病学与精神病学]