复方黄芪有效部位对冠脉结扎致缺血大鼠心肌细胞超微结构的影响  

Effect of HQSM on Cardiac Muscle Cell Ultrastructure of Ischemic Rats Induced by Ligating Coronary Artery

作　　者：陈素红[1] 吕圭源[2] 王丽霞[2] 李立文[2] 张涵[2] 楼招欢[2] 张晓东[2] 吴晶[2] 

机构地区：[1]温州医学院,浙江温州325035 [2]浙江中医药大学,杭州310053

出　　处：《中药新药与临床药理》2006年第6期399-401,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家十五重大科技攻关专项(863计划;2003AA223254)。

摘　　要：目的观察复方黄芪有效部位(HQSM)对冠脉结扎致缺血大鼠心肌细胞形态学及超微结构的影响。方法将SD大鼠随机分成5组：假手术组、模型对照组、黄芪生脉饮组、HQSM高、低剂量组。每天灌胃给药1次,连续30 d。末次给药后2 h,结扎左冠状动脉造成急性心肌缺血模型。描记心电图,连续观察6 h。取心肌组织,于光镜下观察心肌组织的形态学、透射电镜下观察心肌细胞的超微结构。结果光镜下心肌组织形态学：HQSM能改善冠脉结扎致缺血心肌细胞的形态学变化,肌纤维排列较整齐,横纹较清晰。透射电镜下心肌细胞超微结构：HQSM能减轻冠脉结扎所致缺血心肌细胞的超微结构损害,心肌细胞明暗带清晰,肌丝排列基本有序,线粒体轻度肿大。结论HQSM对冠脉结扎所致心肌缺血大鼠的心肌细胞变化具有改善作用。Objective To observe the effect of active part HQSM of Compound Huangqi on the pathological changes and uhrastructure of cardiac muscle cell in ischemic rats induced by ligating coronary artery. Methods SD rats were randomly divided into five groups： sham - operated group, model group, Huangqi Shengmai Yin group, high - and low - dosage HQSM groups. The drug was administrated by gavage once a day for 30 days. The left anterior descending branch of coronary artery was ligated to make the model of acute myocardial ischemia 2 h after the last administration. The electrocardiogram was recorded for 6 hours. Then the morphology of cardiac tissue was observed under light microscope, and the ultrastructure of cardiocyte was observed under transmission electron microscope. Results Myocardial tissue morphological changes under light microscope were as follows： HQSM can improve the morphological changes of ischemic myocardial cells caused by ligating coronary artery, the cardiac muscle fibers were arranged in order, and the transverse striation was distinct. Uhrastructure of cardiac muscle cell under transmission electron microscope were as follows： HQSM can obviously relieve the damage of myocardial uhrastructure, light bond and dark bond of cardiac muscle cell were clear, myofil ament aligned in order, and chondriosome was swollen slightly. Conclusion HQSM can improve the changes of cardiac muscle cell induced by myocardial ischemia through ligating coronary artery.

关 键 词：复方黄芪有效部位 心肌缺血 心肌细胞 疾病模型 动物 

分 类 号：R285.5[医药卫生—中药学]