胡杨树皮多酚的提取工艺研究  

Study on the Extraction Process of Polyphenols from the Bark of Populus euphratica

作　　者：刘松[1] 廖蓉苏[1] 李俊清[2] 

机构地区：[1]北京林业大学理学院,北京100083 [2]北京林业大学资源与环境学院,北京100083

出　　处：《生物质化学工程》2006年第6期13-16,共4页Biomass Chemical Engineering

基　　金：国家自然科学基金资助项目(30471369)

摘　　要：在单因素试验的基础上,采用正交试验,以普鲁士蓝法测定多酚含量为考察指标,探讨了胡杨中植物多酚的提取工艺。由极差分析得知,在本试验所考察的影响因素中,提取时间的影响最大,其次是固液比,溶剂体积分数次之,提取温度对提取率的的影响最小。结果表明:胡杨中植物多酚提取的最佳试验条件为:提取溶剂为40%的丙酮水溶液,固液比为1∶7,提取时间为3 h,提取温度为45℃,并结合单因素试验,确定提取次数为3次。在上述条件下,测得胡杨树皮中多酚含量为6.10%,提取率达到99.03%,平均回收率为94.64%。On the basis of single factor test, the extraction process of polyphenols from Populus euphratica was studied by orthogonal test through the polyphenols content determined by Prussian Blue spectrophotometry. From the variance analysis, the order influenced extraction of polyphenols was extraction time 〉 ratio of solid to liquid 〉 volume fraction of solvent 〉 extraction temperature. The results showed that the optimal extraction conditions were 40 % acetone aquatic solution as extractant, 1 ： 7 of the ratio of solid to liquid,3 h of extraction time, 45℃ of extraction temperature and 3 times of extraction. Under above optimal conditions, it was found that the polyphenols content in Populus euphratica bark was 6.10 %, the extraction rate of polyphenols was 99.03 %, and the average recovery of polyphenols was 94.64 %.

关 键 词：正交试验 胡杨 植物多酚 

分 类 号：TQ914.1[化学工程]