A New Technique of Hepatic Ischemia in the Rat  

作　　者：Yuxin Chen Lei Zhang Zhixin Meng 

机构地区：[1]Department of General Surgery, Qilu Hospital, Shandong University, Jinan, Postcode： 250012.

出　　处：《Journal of US-China Medical Science》2006年第2期26-29,共4页美中医学（英文版）

基　　金：Supported by the Natural Science Foundation of China （No.30371357）.

摘　　要：A new ischemia model is described combining partial hepatectomy with clamping the remnant liver. Three clamps were placed across the pedicles of the median and left lateral lobe at the level of the hilum to achieve total ischemia after the resection of the caudate, right lateral, quadrate lobes, and papillary process. Mesenteric congestion was prevented with the spontaneous portal-caval shunts through the caudate branches and collateral vessels. Survival rate was studied following 60, 90 and 120 minutes ischemia. Liver function was tested. All rats survived over the resection of the liver tissues, and the procedure took 23 ± 3 minutes, and no bleeding of the resected tissue was observed. Spontaneous portal-caval shunts through the caudate branches and collateral vessels were demonstrated by the use of an intraportal dye. Sixty percent of animals survived 90 minutes of ischemia. This model is technically simple and applicable with an anhepatic period during ischemia and without the use of extra portal-systemic shunts.

关 键 词：liver ischemia RAT 

分 类 号：R511[医药卫生—内科学]