配体交换毛细管区带电泳拆分未衍生化氨基酸  

Chiral Separation of Underivatized Amino Acids by Ligand-exchange Capillary Zone Electrophoresis

作　　者：赵艳芳[1] 赵亮[1] 明永飞[1] 李秀娟[1] 蒋生祥[1] 李永民[1] 

机构地区：[1]中国科学院兰州化学物理研究所

出　　处：《分析化学》2006年第U09期87-90,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20375045);中国科学院"西部之光"资助项目(2003)

摘　　要：以铜(Ⅱ)-L-谷氨酸络合物为手性分离选择剂,对苯丙氨酸、酪氨酸和色氨酸3种非衍生芳香族氨基酸的手性对映体拆分进行了研究,建立了一种快速、简便拆分未衍生化的氨基酸对映体的配体交换毛细管电泳方法。在使用10mmol/LNH4AC(pH5·0),5mmol/LCuSO4和10mmol/LL-谷氨酸的条件下,成功地拆分了苯丙氨酸、酪氨酸手性对映体;色氨酸手性对映体也得到部分分离;考察了电泳缓冲液组成、pH值等影响分离效果的因素。A ligand-exchange capillary electrophoretic method was explored, with L-glutamic acid as the ligand and copper （Ⅱ） as the central ion. Its applicability was demonstrated with underivatized aromatic amino acids, namely D,L-phenylalanine, D,L-tyrosine and D,L-tryptophan. Optical resolutions of a single pair of amino acid enantiomers were obtained with a running buffer of 10 mmol/L NH4AC, 5 mmol/L Cu（Ⅱ） and 10 mmol/L L-glutamic acid at pH 5.0. The resolution was found to increase with the concentration of the Cu（Ⅱ） complex at a Cu（Ⅱ） to glutamic acid ratio of 1：2. The pH of buffer was another important factor controlling the separations. For all the studied amino acids, the optimum pH was 5.0.

关 键 词：手性配体毛细管电泳 手性分离 L-谷氨酸 氨基酸 

分 类 号：O658.9[理学—分析化学]