灵芝子实体中两个新的天然三萜类化学成分的分离、纯化和鉴定  

Isolation,purification and identification of two new triterpenoid constituents from the fruiting bodies of Ganoderma lucidum

作　　者：郝瑞霞[1] 张劲松[2] 唐庆九[2] 胡立宏[3] 郑宗平[3] 潘迎捷[4] 

机构地区：[1]南京农业大学生命科学学院,南京210095 [2]上海市农业科学院食用菌研究所,上海201106 [3]中国科学院上海药物研究所,上海201203 [4]上海水产大学食品科学学院,上海200090

出　　处：《菌物学报》2006年第4期599-602,共4页Mycosystema

基　　金：上海市农委重点攻关项目(No.农科攻字2002-1-4-3)

摘　　要：本文采用硅胶和MCI柱层析的方法,从灵芝Ganodermalucidum子实体中分离纯化三萜类化合物。从灵芝子实体的氯仿萃取层中,分离纯化到灵芝属中的2个新天然产物,运用现代NMR技术分析确定了它们的结构,分别为methyl7β-hydroxy-3,11,15,23-tetraoxo-5α-lanost-8-en-26-oate(methylganoderateD)(Ⅰ)和methyl12β-acetoxy-3,7,11,15-tetraoxo-5α-lanost-8-en-24-oate(methyllucidenateD)(Ⅱ)。The triterpenoid constituents from the fruiting bodies of Ganoderma lucidum were isolated and purified by means of column chromatography. Two new natural products from Ganoderma fruiting bodies were obtained from the chloroform extract. Their structures were identified as methyl 7 β-hydroxy-3, 11, 15, 23- tetraoxo-5α-lanost-8-en-26-oate （methyl ganoderate D） （I） and methyl 12β-acetoxy- 3, 7, 11, 15- tetraoxo- 5α- lanost- 8-en-24-oate （methyl lucidenate D ） （II）on the basis of their NMR spectral data.

关 键 词：生物活性成分 核磁共振 化学结构 

分 类 号：R284[医药卫生—中药学]