Rel mRNA和NF-κB2 mRNA在人肝细胞癌中的表达及其临床意义  

Expression and Significance of Rel mRNA and NF-κB2 mRNA in Hepatocellular Carcinoma

作　　者：陈远能[1] 曹骥[2] 苏建家[2] 李瑗[2] 朱振宇[3] 

机构地区：[1]广西医科大学附属第一医院消化内科,广西南宁520021 [2]广西医科大学附属肿瘤医院实验研究部,广西南宁520021 [3]中山大学中山医学院生化教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2006年第6期677-680,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(39860072);广西壮族自治区回国人员自然科学基金资助项目(199817137)

摘　　要：【目的】探讨Rel及NF-κB2的mRNA在人肝细胞癌(hepatocellularcarcinoma,HCC)组织中的表达及其临床意义。【方法】用逆转录聚合酶链反应检测55例HCC患者癌组织及癌旁肝组织中RelmRNA和NF-κB2mRNA的表达情况。【结果】RelmRNA及NF-κB2mRNA在肝癌组织中的表达水平均明显低于其癌旁组织(分别为0.315±0.142对0.505±0.225;0.273±0.141对0.491±0.242,均P<0.05)。这两种因子在肝癌组织中的表达水平与临床分期及肝外转移明显有关,而与门静脉癌栓、术后复发、肿瘤数目、肿瘤直径及血清AFP水平等无明显关系。【结论】提示Rel、NF-κB2可能与肝癌的发生、发展有关。[Objective] The aims of the present study were to explore the expression of the Rel mRNA and NF-κB2 mRNA in human hepatocellular carcinoma （HCC） and their clinical significance. [Methods] Reverse transcription polymerase chain reaction （RT-PCR） was employed to determine the expression of Rel mRNA and NF-κB2 mRNA in 55 cases of HCC and their adjacent liver tissues. [Results] The expression level of Rel mRNA and NF-κB2 mRNA in the HCC tissues were significantly lower than that in the adjacent liver tissues （0.315 ± 0.142 vs 0.505 ± 0.225 and 0.273 ± 0.141 vs 0.491 ± 0.242, respectively, P〈 0.05）. The expression level of Rel mRNA and NF-κB2 mRNA in the HCC tissue were significantly correlated with the clinical stage and the presence of extrahepatic metastasis, but not correlated with the tumor thrombus of portal vein, the tumor recurrence, the number of tumor focus, the tumor diameter and the level of serum alpha-fetoprotein （AFP）. [Conclusion] The results suggest that NF-κB2 and Rel may be involved in the development of HCC.

关 键 词：REL MRNA NF-κB2 MRNA 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]