中国吐鲁番两株产木聚糖酶的极端耐碱Bacillus halodurans的分类鉴定(英文)  

Isolation and identification of two xylanase_producing extremely alkali_tolerant strains of Bacillus halodurans from Turpan in China

作　　者：易霞[1] 谢周杰[2] 邓爱华[1] 王宁[1] 艾尔肯.热合曼 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2006年第6期951-955,共5页Acta Microbiologica Sinica

基　　金：国家教育部重点科研项目(03149) ;自治区重点实验室开放课题(XJYS0203-2004-01)~~

摘　　要：通过生理生化实验、16S rDNA序列分析和同源性杂交，将分离到的XJU-1和XJU-80菌种进行了分类鉴定。XJU-1和XJU-80具有较宽的pH生长范围（分别是pH4．5～12．6和pH3．8～12．6），其G＋C mol％含量分别是40．5mol％和42．2mol％。16S rDNA序列分析和DNA-DNA同源杂交结果表明，XJU-1和XJU-80与Bacillus halodurans C-125和Bacillus halodurans DSM497^T具有较高的同源性（99％）；两者之间也具有85％的相关性，但其与Bacillus halodurans C-125和Bacillus halodurans DSM497^T分别具有81．3％和71．5％的相关性。基于以上结果，将两株分离菌株分类为Bacillus halodurans的两个新品系。Bacillus halodurans XJU-1 and XJU-80 were characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA-DNA hybridization analysis. The two isolates can grow in nutrient broth at a broad range of pH values from 4.5 to 12.6 for XJU- 1 and from 3.8 to 12.8 for XJU-80, respectively. And the optimum temperature of growth were around 39℃ and 42℃, respectively. Phylogenetic analysis of the two strains based on comparison of 16S rRNA sequence revealed that they are closely related to Bacillus halodurans C-125 and DSM49^Twith 99% identity. DNA-DNA hybridization showed that the highest levels of DNA-DNA relatedness were found between the two strains （85%） and the B. halodurans type strains （81.3% and 71.5% ）, respectively. Moreover, the G ＋ C content of the genomic DNA was 40.5 mol% for XJU-1 and 42.2 mol% for XJU-80.Our results demonstrate that strains XJU-1 and XJU-80 should be classified as two new members of the species B. halodurans.

关 键 词：BACILLUS halodurans G+C mol%含量 16S RRNA DNA-DNA同源杂交 进化树分析 

分 类 号：Q93-33[生物学—微生物学]