甲基对硫磷彻底降解菌X4的分离、降解性及系统发育研究  

Isolation,degradation and phylogenetic analysis of methylparathion degradative strain X4

作　　者：解秀平[1] 闫艳春[2] 刘萍萍[1] 

机构地区：[1]山东农业大学生命科学学院作物生物学国家重点实验室,泰安271018 [2]中国农业科学院研究生院,北京100081

出　　处：《微生物学报》2006年第6期979-983,共5页Acta Microbiologica Sinica

摘　　要：从生产甲基对硫磷的山东华阳农药厂污水曝气池中,分离到一株能以甲基对硫磷及其降解中间产物对硝基苯酚为唯一碳源生长,且能够将其彻底降解为CO2和H2O的细菌X4,经鉴定,为节杆菌属(Arthrobactersp.)。用气相色谱法和分光光度法对X4的降解性能分析表明,X4在7h内对50mg/L甲基对硫磷、50mg/L对硝基苯酚的降解率为99%以上,对其它有机磷农药也有良好的降解效果,测定条件为:pH值7,温度30℃,接种量30%。并构建了系统发育树以了解其它菌株与X4之间的亲缘关系。The strain X4 was isolated from polluted wastes of Shandong Huayang Pesticide Chemical Industry Group Co., Ltd. It was cultured with methylparathion and ρ-nitrophenol, a kind of intermediate during methylparathion degrading process, and used them as the sole carbon source and decomposed them into carbon dioxide and wastes. It is identified as Arthrobacter sp. by means of a series of tests, and it has visible sphere-pole variable cycle. It is bacilliform when it is cultured in nutrient broth medium within 36h, then it become short gradually, and ultimately become uniform sphericity. Bacilliform strains array in ＂V＂ or ＂Y＂ shape. It has flagellum, moveable, no sporangium, oxidates glucose, The degradative ability to pesticides of X4 was determined by gas chromatography （GC） and spectrophotometer, and the degradative rate for methylparathion at 50mg/L and ρ- nitrophenol at 50mg/L is above 99% within 7h. Moreover, X4 also can degrade other organophosphate pesticides quickly. The degradative rate for methamidophos, chlorpyrifos and carbofuran is 64%, 54% and 68%, respectively. The maximal tolerant concentration of X4 to MP, PNP, methamidophos, chlorpyrifos and carbofuran is 1700mg/L, 500mg/L, 500mg/L, 300mg/L and 300mg/L, respectively. All of the above tests were determined at 30℃, pHT, with an inoculum of 30%. At the same time, 16S rRNA sequence of X4 is obtained, and the similar sequences to X4 were selected from GenBank by BLAST program and the phylogenetic tree with these sequences was constructed, the relationship between X4 and referenced strains was worked out. At present, methylparathion and ρ-nitrophenol degradative strains Alcaligenes sp. and so on. Furthermore, those strains can only reported are mostly Pseudomonas sp., Flavobacterium, degrade MP and PNP partially. It is reported firstly here that Arthrobacter sp. can degrade both MP and PNP to small molecule synchronously and effectively. Finally, the strain X4 shows a huge potential to be used in a bioremediation application for the treat

关 键 词：甲基对硫磷 对硝基苯酚 节杆菌 降解 系统发育树 

分 类 号：X172[环境科学与工程—环境科学]