年龄对大鼠骨髓内皮祖细胞数量和功能的影响及其意义  

Relationship between aging and the number and function of bone marrow-derived endothelial progenitor cells in rats

作　　者：陈剑飞[1] 黄岚[1] 晋军[1] 武晓静[1] 刘兰[1] 周健[1] 赵晓晖[1] 崔斌[1] 

机构地区：[1]第三军医大学新桥医院全军心血管内科中心,重庆400037

出　　处：《中华心血管病杂志》2006年第11期1026-1028,共3页Chinese Journal of Cardiology

基　　金：国家自然科学基金资助项目(30400517;30470729)

摘　　要：目的观察不同年龄大鼠骨髓内皮祖细胞(endothelial progenitor cells,EPC)的数量和功能,探讨年龄对EPC功能与活性的影响。方法选择4月龄、10月龄、18月龄SD大鼠各10只,用密度梯度离心法从骨髓获取单个核细胞,将其接种于培养板,培养7天后对贴壁细胞进行细胞化学分析。激光共聚焦显微镜鉴定DiI标记的乙酰化低密度脂蛋白(DiI-acLDL)和FITC标记荆豆凝血素Ⅰ(FITC-UEA-Ⅰ)双染色阳性细胞为正在分化的EPC。采用改良的Boyden小室和黏附能力测定实验观察EPC的迁移和黏附能力。结果随年龄的增加大鼠骨髓EPC数量明显减少,4月龄、10月龄、18月龄分别为(42.0±5.8)、(30.8±4.7)、(21.5±4.7)EPC/视野(×400),组间比较P<0.01,且高龄大鼠骨髓EPC的黏附和迁移能力明显受损。结论年龄增加可导致大鼠骨髓EPC数量减少,并使EPC迁移、黏附功能减退。Objective To investigate the relationship between aging and the number and function of bone marrow-derived endothelial progenitor cells （EPC） in rats. Methods The number and function of bone marrow-derived EPC were measured in 4, 10 and 18 months old rats （n = 10 in each group）. Mononuclear cells （MNC） were isolated from rat bone marrow by Ficoll density gradient centrifugation and cultured for 7 days,EPC were identified as adherent cells double positive stained for FITC-UEA-Ⅰ and DiI-acLDL under laser confocal immunofluence microscopey. Boyden chamber was used to determine the migration capacity of EPC and the number of recultured EPC was counted to test the adherent function of EPC. Result The number （42.0 ±5.8 vs 30. 8 ±4. 7 vs 21.5 ±4. 7 per field） and the migration and adhesion capacities of EPC were significantly reduced with aging. Conclusion This study shows that the number and function of EPC are decreased with aging.

关 键 词：干细胞 骨髓 年龄因素 大鼠 

分 类 号：R363[医药卫生—病理学]